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目的:探讨黄芩茎叶的抗菌活性物质基础。方法:60%乙醇冷浸提取,通过硅胶柱色谱,聚酰胺柱色谱,羟丙基葡聚糖凝胶(Sephadex LH-20)柱色谱及HPLC,重结晶等方法分离纯化,根据化合物的理化性质和波谱数据(1H-NMR,13CNMR,UV,MS)鉴定化学结构。结果:从黄芩茎叶60%乙醇提取物中,分离得到8个单体化合物,分别鉴定为:芹菜素-7-O-β-D-葡萄糖醛酸苷(1),5,4’-二羟基-7-甲氧基二氢黄酮(2),芫花素(3),千层纸素A-7-O-β-D-葡萄糖醛酸苷(4),7-甲氧基白杨素(5),白杨素(6),3’,4’,5,5’,7-五甲氧基黄酮(7),红花素-7-O-β-D-葡萄糖醛酸苷(8)。结论:化合物3为首次从黄芩茎叶中分离得到,化合物2,5,7为首次从黄芩植物中分离得到。
Objective: To explore the basis of antimicrobial activity of stems and leaves of Scutellaria baicalensis. METHODS: Cold-extracted with 60% ethanol and purified by column chromatography on silica gel, polyamide column, Sephadex LH-20, HPLC and recrystallization. According to the physicochemical properties And spectroscopic data (1H-NMR, 13CNMR, UV, MS). Results: Eight monomer compounds were isolated from 60% ethanol extract of stems and leaves of Scutellaria baicalensis and identified as apigenin-7-O-β-D-glucuronide (1), 5,4’- Hydroxy-7-methoxyflavanone (2), genkwanin (3), melaleuca A-7-O-β-D-glucuronide (4) (5), chrysin (6), 3 ’, 4’, 5,5 ’, 7-pentamethoxyflavone (7), safflor-7-O-β-D-glucuronide ). CONCLUSION: Compound 3 was isolated from stems and leaves of Scutellaria baicalensis for the first time. Compounds 2, 5, and 7 were isolated from Scutellariae for the first time.