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以三聚磷酸钠作为交联剂,采用离子交联法制备了不同构成的水溶性壳聚糖纳米粒子(WSCNP).以牛血清蛋白(BSA)为模型药物,所制得的空载及载药WSCNP粒径、Zeta电位分别在35～190nm和35～42mV之间.红外光谱证实了纳米粒子中水溶性壳聚糖的氨基与TPP的磷酸基团发生了交联反应.考察了WSCNP蛋白药物释放的一些影响因素.BSA浓度的增加(0.05～1mg/mL)提高了WSC的载药量但同时降低了负载率;聚乙二醇的添加加速了WSC载体中BSA的释放;WSC的脱乙酰度(72.6%～90%)及分子量(3.5～15.8kDa)的增加在一定程度上提高了负载率而降低了释放率.结果表明,水溶性壳聚糖是一种极具应用潜力的蛋白药物释放载体.
Water-soluble chitosan nanoparticles (WSCNP) with different compositions were prepared by ion-crosslinking method using sodium tripolyphosphate as crosslinking agent.With bovine serum albumin (BSA) as a model drug, The particle size and Zeta potential of drug WSCNP were between 35 ~ 190nm and 35 ~ 42mV, respectively.The infrared spectra confirmed that the amino groups of the water-soluble chitosan in the nanoparticles cross-linked with the phosphate group of TPP.We investigated the effect of WSCNP protein drug (0.05 ~ 1mg / mL) increased the drug loading of WSC but at the same time reduced the loading rate; the addition of PEG accelerated the release of BSA in WSC carrier; the deacetylation of WSC Degree (72.6% ~ 90%) and molecular weight (3.5 ~ 15.8kDa) increased to a certain extent, increased the loading rate and reduced the release rate.The results showed that water-soluble chitosan is a potential application of protein drugs Release vector.