论文部分内容阅读
目的改进嗜水气单胞菌J-1株灭活疫苗的生产工艺,提高培养效率。方法采用改良培养基和产毒素培养基,利用机械式通风搅拌培养J-1菌株,确定发酵培养时间,并比较两种培养基的培养效果;用摇瓶灭活和罐灭活J-1菌株培养液,比较不同浓度甲醛的灭活效果。结果用机械搅拌发酵罐培养J-1菌株,菌体生长12～14 h达峰值,产毒素培养基菌液活菌数平均为102×108 CFU/ml,改良培养基活菌数平均为216×108 CFU/ml,较产毒素培养基提高了111.7%,活菌数最大可达300×108 CFU/ml;改良培养基培养J-1菌株,培养液毒力略高于产毒素培养基;0.3%的福尔马林于37℃灭活24 h,可完全灭活J-1菌株培养液,且制备的灭活疫苗福尔马林残留量符合质量标准。结论改良培养基培养J-1菌株的效果明显优于产毒素培养基,机械式搅拌发酵罐培养嗜水气单胞菌,可比原工艺缩短培养时间一半。
Objective To improve the production process of Aeromonas hydrophila strain J-1 inactivated vaccine and improve the culture efficiency. Methods The modified culture medium and toxin-producing medium were used to culture J-1 strain by mechanical ventilation and stirring to determine the fermentation time and to compare the culture effects of the two culture media. Inactivated J-1 strain Culture medium, compare the inactivation effect of different concentrations of formaldehyde. Results The strain J-1 was cultured in a mechanically stirred fermentor. The number of viable cells in the toxin-producing medium was 102 × 108 CFU / ml and the average number of viable cells in the modified medium was 216 × 108 CFU / ml, which was 111.7% higher than that of the toxin-producing medium and the viable count was up to 300 × 108 CFU / ml. The strain J-1 was cultured in modified medium, % Of formalin at 37 ℃ inactivated 24 h, the complete inactivation of J-1 strain culture medium, and the prepared inactivated vaccine formalin residues meet the quality standards. Conclusion The modified culture medium is superior to that of the toxin-producing medium for culturing J-1 strain. Aeromonas hydrophila can be cultured in a mechanical stirring fermenter, which can shorten the culturing time by half compared with the original technology.