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以黄瓜高抗白粉病品种JIN5-508为供体亲本,以高感白粉病品种D8为轮回亲本,通过多代回交后自交,并在每个世代结合白粉病抗性进行接种鉴定,获得了17个以D8为遗传背景但具有抗白粉病的染色体片段导入系。利用平均分布于黄瓜7条染色体上的324对SSR引物对17个导入系63个抗病单株(病情指数≤10)的染色体导入片段特征进行了鉴定。结果表明:74对SSR引物在亲本间表现多态性;63个抗病单株中单片段导入系共25株,占39.68%;导入双片段的共32株,占50.79%;有3个供体片段导入的为4株,占6.35%;无片段导入的2株,占3.17%。出现频率较高的导入片段共4个,分别位于1号染色体的23.4~41.3cM、3号染色体的51.1~54.0cM、4号染色体的9.3~11.3cM和6号染色体的1.4~3.2cM处,出现的频率分别为74.6%、44.4%、25.4%和7.9%。
The cucumber highly resistant to powdery mildew variety JIN5-508 was used as the donor parent, and the highly susceptible powdery mildew variety D8 was used as the reincarnated parent, selfed through multiple generations of backcrossing and inoculated with powdery mildew resistance in each generation for identification A total of 17 chromosome segments with D8 genetic background but resistance to powdery mildew were introduced. A total of 17 pairs of SSR primers were used to identify the chromosomally introduced fragments of 63 resistant plants (disease index ≤10) in 17 inbred lines using 324 pairs of SSR primers evenly distributed on 7 chromosomes of cucumber. The results showed that 74 pairs of SSR primers showed polymorphism among the parents. A total of 25 strains (39.68%) were introduced into the 63 single disease-resistant plants. A total of 32 strains (50.79%) were introduced into the double-fragment. Four of them were introduced into the body fragment, accounting for 6.35%. Two of them were introduced without fragment, accounting for 3.17%. A total of 4 introduced fragments were located at 23.4 ~ 41.3cM on chromosome 1, 51.1 ~ 54.0cM on chromosome 3, 9.3 ~ 11.3cM on chromosome 4 and 1.4 ~ 3.2cM on chromosome 6, respectively. The frequency of occurrence was 74.6%, 44.4%, 25.4% and 7.9% respectively.