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目的 :以沙苑子苷为对照品测定沙苑子总黄酮含量。方法 :80 %乙醇提取液 ,2 6 6nm测定紫外吸收度。结果 :总黄酮含量为 2 1.5mg/g ,RSD =1.0 0 % ;回收率 95 .4 % ,RSD =2 .2 7% ,线性范围 4～ 2 4ug/ml。结论 :本法测定沙苑子总黄酮含量 ,操作简便 ,稳定性好 ,可用于沙苑子的质量控制。
Objective : To determine the content of total flavonoids in Astragalus sp. Method : 80% ethanol extract, 260 nm absorbance was measured. Results: The content of total flavonoids was 2 1.5mg/g, RSD was 1.00%, the recovery was 95.4 %, RSD was 2.7%, and the linear range was 4～24ug/ml. Conclusion : The method can be used to determine the total flavonoid content of Astragalus complanatus with simple operation and good stability. It can be used for the quality control of Astragalus complanatus.