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目的:建立一种同时测定六味地黄浓缩丸中丹皮酚、芍药苷、马钱苷、没食子酸4种活性成分含量的方法。方法:应用高效液相法进行测定活性成分的含量,色谱柱为Alltima C18柱(250 mm×4.6 mm);流动相为乙腈-0.1%甲酸梯度洗脱;流速为0.5 mL/min;检测波长为242 nm;进样量5μL;柱温为30℃。结果:丹皮酚、芍药苷、马钱苷、没食子酸线性范围分别为0.199～3.98μg(r=0.999 8)、0.073～1.172μg(r=0.999 5)、0.145～2.316μg(r=0.999 5)、0.103～1.648μg(r=0.999 4);平均加样回收率分别为99.35%(RSD=1.22%)、98.16%(RSD=1.57%)、97.30%(RSD=1.77%)、98.96%(RSD=1.16%)。结论:本法方便、稳定、可靠,可用于六味地黄浓缩丸的质量控制。
OBJECTIVE: To establish a method for the simultaneous determination of four active ingredients of paeonol, paeoniflorin, loganin and gallic acid in Liuwei Dihuang Pills. Methods: The content of active ingredient was determined by HPLC. The column was Alltima C18 column (250 mm × 4.6 mm). The mobile phase consisted of acetonitrile-0.1% formic acid gradient. The flow rate was 0.5 mL / min. The detection wavelength was 242 nm; injection volume 5μL; column temperature was 30 ℃. Results: The linear range of paeonol, paeoniflorin, loganin and gallic acid were 0.199 ~ 3.98μg (r = 0.999 8), 0.073 ~ 1.172μg (r = 0.999 5), 0.145 ~ 2.316μg (RSD = 1.22%), 98.16% (RSD = 1.57%), 97.30% (RSD = 1.77%) and 98.96% (RSD = 1.999% RSD = 1.16%). Conclusion: This method is convenient, stable and reliable and can be used for quality control of Liuwei Rehmannia glutin concentrate pills.