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在分子水平阐明细胞生理活动深层次的机制是当前生命科学的重要研究课题.AFM的发明为揭示细胞生理活动的分子本质提供了新的技术手段.利用AFM单分子力谱技术在近生理环境下对B淋巴瘤细胞表面的CD20抗原与其抗体Rituximab之间的特异性结合反应进行了探索性的研究,通过对探针进行功能化,测量了CD20抗原与Rituximab之间的特异性结合力,同时观察了CD20抗原在B淋巴瘤细胞表面的分布,并分析了在外部拉力作用下,CD20-Rituximab复合物的分子内力与伸长量的关系.实验结果为深入研究Rituximab的作用机制奠定了基础.
At the molecular level to clarify the underlying mechanism of cellular physiology activities is an important research topic in current life sciences.AFM invention provides a new technical means to reveal the molecular nature of cell physiological activity.Using AFM single molecule force spectroscopy in near physiological environment The specific binding reaction between the CD20 antigen on the surface of B lymphoma cells and its antibody Rituximab was explored. By functionalizing the probe, the specific binding capacity between CD20 antigen and Rituximab was measured and observed The distribution of CD20 antigen on the surface of B lymphoma cells was analyzed and the relationship between molecular internal force and elongation of CD20-Rituximab complex under external tension was analyzed.The experimental results laid the foundation for further study on the mechanism of Rituximab.