论文部分内容阅读
本试验旨在研究维生素B1、维生素B2不同摄入量对12月龄伊犁马血液、尿液相关指标的影响,以得到12月龄伊犁马对维生素B1、维生素B2的需要量。试验选取12月龄±5日龄、平均体重(245.28±18.36)kg的伊犁马公马35匹,随机分为5组,每组7匹,分别为试验Ⅰ组、试验Ⅱ组、试验Ⅲ组、试验Ⅳ组、试验Ⅴ组,马匹饲喂相同的基础饲粮,并分别添加0、16、32、48和64 mg/(匹·d)的维生素B1和0、10、20、30和40 mg/(匹·d)的维生素B2,进行为期20 d的饲养试验。试验Ⅰ组、试验Ⅱ组、试验Ⅲ组、试验Ⅳ组、试验Ⅴ组实际摄入维生素B1量分别为19.00、33.49、48.27、62.96、77.53 mg/(匹·d),实际摄入维生素B2量分别为21.95、31.68、41.77、51.53、61.26 mg/(匹·d)。结果表明:随着实际摄入维生素B1量的增加,伊犁马血浆维生素B1含量、红细胞转酮酶活性(E-TKA)、尿液维生素B1排出量逐渐升高,焦磷酸硫胺素(TPP)效应逐渐降低。试验Ⅰ组血浆维生素B1含量显著低于试验Ⅲ组(P<0.05),极显著低于试验Ⅳ组、试验Ⅴ组(P<0.01);试验Ⅰ组E-TKA极显著低于试验Ⅲ组、试验Ⅳ组和试验Ⅴ组(P<0.01);试验Ⅰ组TPP效应显著高于试验Ⅲ组(P<0.05),极显著高于试验Ⅳ组、试验Ⅴ组(P<0.01);试验Ⅰ组尿液维生素B1排出量极显著低于试验Ⅲ组、试验Ⅳ组、试验Ⅴ组(P<0.01)。随着实际摄入维生素B2量的增加,伊犁马血浆维生素B2含量出现波动变化,红细胞谷胱甘肽还原酶活性系数(E-GRAC)逐渐降低,尿液维生素B2排出量逐渐增加。试验Ⅰ组血浆维生素B2含量显著低于试验Ⅲ组、试验Ⅳ组(P<0.05),极显著低于试验Ⅱ组、试验Ⅴ组(P<0.01);试验Ⅰ组E-GRAC极显著高于试验Ⅱ组、试验Ⅲ组、试验Ⅳ组、试验Ⅴ组(P<0.01);试验Ⅰ组尿液维生素B2排出量极显著低于试验Ⅲ组、试验Ⅳ组、试验Ⅴ组(P<0.01)。综合评价各指标,得到12月龄伊犁马对维生素B1的需要量为48.27 mg/(匹·d),对维生素B2的需要量为31.68 mg/(匹·d)。
The purpose of this study was to investigate the effect of different intake of vitamin B1 and vitamin B2 on blood and urine related indicators of 12-month-old Ili animals to get the requirement of vitamin B1 and vitamin B2 for 12-month-old Ili. A total of 35 Ili horses aged 12 months ± 5 days and average body weight 245.28 ± 18.36 kg were randomly divided into 5 groups with 7 rabbits in each group as experimental group Ⅰ, group Ⅱ, group Ⅲ, In group IV and group V, the horses were fed the same basal diet and supplemented with 0, 16, 32, 48 and 64 mg / (d · d) of vitamin B1 and 0, 10, 20, 30 and 40 mg / (P · d) of vitamin B2, for a period of 20 d feeding test. The actual amount of intake of vitamin B1 in group Ⅰ, group Ⅱ, group Ⅲ, group Ⅳ and groupⅤ was 19.00, 33.49, 48.27, 62.96 and 77.53 mg / (d · d) respectively. The actual amount of intake of vitamin B2 Respectively 21.95,31.68,41.77,51.53,61.26 mg / (P · d). The results showed that with the increase of actual intake of vitamin B1, the content of vitamin B1, the activity of E-TKA and the excretion of vitamin B1 in the urine of Yili horse were gradually increased. The content of thiamine pyrophosphate (TPP) The effect is gradually reduced. The content of vitamin B1 in group Ⅰ was significantly lower than that in group Ⅲ (P <0.05), significantly lower than that in group Ⅳ (P <0.01). The level of E-TKA in group Ⅰ was significantly lower than that in group Ⅲ (P <0.01). The TPP effect in test group I was significantly higher than that in test group Ⅲ (P <0.05), significantly higher than that in test group Ⅳ and test group Ⅴ (P <0.01) The excretion of vitamin B1 in urine was significantly lower than that in group Ⅲ, group Ⅳ and groupⅤ (P <0.01). As the actual intake of vitamin B2 increased, the content of vitamin B2 in the plasma of Ili horse was fluctuated, the activity coefficient of erythrocyte glutathione reductase (E-GRAC) gradually decreased and the amount of vitamin B2 excretion increased gradually. The content of vitamin B2 in test group Ⅰ was significantly lower than that in test group Ⅲ, and in test group Ⅳ (P <0.05), it was significantly lower than that in test group Ⅱ and test group Ⅴ (P <0.01) (P <0.01). The excretion of vitamin B2 in test group Ⅰ was significantly lower than that of test group Ⅲ, test group Ⅳ and test group Ⅴ (P <0.01) . According to the comprehensive evaluation of each index, the 12-month-old Ili horse was required to have vitamin B1 requirement of 48.27 mg / (d · d) and vitamin B2 requirement of 31.68 mg / (d · d).