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1高效液相色谱法:1.1原理试样经处理后,用高效液相色谱氨基柱分离,用示差折光检测器检测,外标法进行测定。1.2试剂除非另有规定,本方法所用试剂均为分析纯。实验用水的电导率(25℃)为0.01mS/m。1.2.1乳糖的标准溶液(50mg/mL)称取在94℃±2℃烘箱内干燥2h的乳糖试样2.5g(精确至0.1mg),溶于水中,用水稀
1 high performance liquid chromatography: 1.1 Principle After the sample is treated, high performance liquid chromatography with amino column separation, refractive index detector detection, external standard method for the determination. 1.2 Reagents Unless otherwise specified, the reagents used in this method are of analytical grade. The conductivity of the experimental water (25 ° C) was 0.01 mS / m. 1.2.1 Standard solution of lactose (50mg / mL) Weigh 2.5g (accurate to 0.1mg) of lactose sample dried in oven at 94 ℃ ± 2 ℃ for 2h, dissolve in water, dilute with water