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枇杷小苗钙营养液沙培试验结果表明:钙胁迫不利枇杷小苗叶片氮代谢的进行,缺钙胁迫在小苗培养60 d时就已经产生影响,而高钙胁迫表现比较滞后,且缺钙胁迫的影响要比高钙处理的大.小苗培养60 d时,0.01 mmol/L、3.2 mmol/L钙胁迫处理会使叶片硝酸还原酶活性增大、O2-净产生速率降低、可溶性蛋白含量增加、脯氨酸含量下降.继续培养至90 d时,3个处理的硝酸还原酶活性都增大、O2-净产生速率上升,胁迫处理对两者的影响与60 d相同;而可溶性蛋白和脯氨酸含量虽急剧下降,但胁迫处理的脯氨酸含量高于对照.小苗培养90 d时,叶片游离氨基酸含量测定结果表明,缺钙处理的游离氨基酸总量和单个氨基酸高于对照,高钙处理低于对照,各处理中单个氨基酸含量最高的是谷氨酸,胱氨酸含量最低.
The results showed that calcium stress was not good for nitrogen metabolism of leaves of loquat seedlings. Calcium deficiency stress had effect on seedling culture at 60 days, but high calcium stress lagged behind, and the effect of calcium deficiency stress Which was higher than that of high-calcium treatment.At 60 days after the seedlings were cultured, 0.01 mmol / L and 3.2 mmol / L Ca2 + treatment increased nitrate reductase activity, O2- net production rate, soluble protein content, proline The content of acid decreased.At 90 days, the activities of nitrate reductase increased and the O2-net production rate increased in the three treatments, and the effect of stress treatment on them was the same as that on the 60th day. The content of soluble protein and proline Although the content of proline decreased sharply, the content of proline in the stress treatment was higher than that in the control.At 90 days after the seedlings were cultured, the content of free amino acids in the leaves was higher than that of the control Control, the highest single amino acid content in each treatment is glutamic acid, cystine content is the lowest.