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目的:探讨间接酶联免疫吸附试验(indirect enzyme-linked immunosorbent assay,IELISA)在诊断人布鲁菌感染中的价值。方法:选取116例布鲁菌病(布病)确诊患者、66例疑似布病患者和72名健康对照者,采集静脉血,应用试管凝集试验(standard tube agglutination test,SAT)、IELISA、虎红平板凝集试验(Rose-Bengal plate agglutination test,RBPT)和血培养方法进行检测,以SAT检测结果为诊断标准,分析IELISA、RBPT和血培养方法在布病诊断中的价值。结果:SAT、IELISA、RBPT及血培养方法检测人血清布鲁菌在急性、慢性布病组中的阳性率(100%、100%;98.6%、97.9%;91.3%、91.5%;20.3%、10.6%)显著高于疑似布病组(0、6.0%、0、0,P均<0.05)及健康对照组(1.4%、0、2.8%、血培养未做,P均<0.01)。IELISA法与RBPT、SAT法间有较好的一致性(Kappa值为0.846和0.966)。IELISA检测的灵敏度(98.3%)和特异度(100%)最高;RBPT法的灵敏度和特异度则分别为91.4%和97.2%。结论:IELISA法具有较高的准确率,可更简便、迅速的对布病进行诊断。
Objective: To investigate the value of indirect enzyme-linked immunosorbent assay (IELISA) in the diagnosis of Brucella infection. Methods: One hundred and sixteen patients with brucellosis (brucellosis), 66 patients with suspected brucellosis and 72 healthy controls were enrolled in this study. Venous blood was collected and the blood samples were collected by standard tube agglutination test (SAT), IELISA, The value of SAT, ITA, RBPT and blood culture methods in the diagnosis of brucellosis was tested by Rose-Bengal plate agglutination test (RBPT) and blood culture methods. Results: The positive rate of Brucella serogroups in acute and chronic brucellosis was detected by SAT, IELISA, RBPT and blood culture methods (100%, 100%; 98.6%, 97.9%; 91.3%, 91.5%; 20.3% 10.6%) was significantly higher than that of the suspected brucellosis group (0,6.0%, 0,0, P <0.05) and healthy control group (1.4%, 0,2.8%, respectively; IELISA method and RBPT, SAT method has good agreement (Kappa value of 0.846 and 0.966). Sensitivity and specificity of IELISA were the highest (98.3%) and specificity (100%) respectively. Sensitivity and specificity of RBPT method were 91.4% and 97.2% respectively. Conclusion: The IELISA method has higher accuracy, which can diagnose brucellosis more easily and rapidly.