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合成反义和正义ｃ－ｍｙｂ１８－ｍｅｒ，分别导入培养的ＷＫＹ主动脉平滑细胞（ＳＭＣｓ），同时用内皮素诱导ＳＭＣｓ增殖。反义ｃ－ｍｙｂ寡核苷酸（ＯＤＮｓ）对内皮素诱导ＳＭＣｓ的抗细胞增殖抑制作用随浓度（６０μｍｏｌ·Ｌ－１～１２０μｍｏｌ·Ｌ－１）的增加而增加。用１２０μｍｏｌ·Ｌ－１反义ＯＤＮＳ抗细胞增殖能力随时间延长而下降。免疫组化显示受抑制细胞ｍｙｂ水平较同期对照组或正义ＯＤＮｓ组低。以上为反义ＯＤＮｓ抑制外源性生长因子或细胞因子诱导靶基因的高表达和细胞增殖提供了新的线索，同时为探讨癌基因表达调控与动脉ＳＭＣｓ增殖的关系提供了一种新方法。
Antisense and sense c-myb18-mer were synthesized and introduced into cultured WKY aorta smooth muscle cells (SMCs) respectively, while the proliferation of SMCs was induced by endothelin. Antisense c-myb oligodeoxynucleotides (ODNs) increased the anti-cell proliferation of endothelin-induced SMCs with increasing concentration (60μmol·L-1 ~ 120μmol·L-1). With 120μmol·L-1 antisense ODNS anti-cell proliferation decreased over time. Immunohistochemistry showed that myb levels in the inhibited cells were lower than those in the control group or the ODNs group. These results provide new clues for the antisense ODNs to inhibit the expression of target genes and cell proliferation induced by exogenous growth factors or cytokines and provide a new method for exploring the relationship between oncogene expression and proliferation of arterial SMCs.