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目的:观察消膏转浊方对高脂饲养的肥胖大鼠脂质过氧化反应以及胰岛素抵抗(IR)的影响。方法:将Wistar雄性大鼠随机分为正常组、模型组、消膏转浊组、罗格列酮组及饮食控制组。正常组以标准饲料喂养,其余组的大鼠饲以高脂饲料。8周后,正常组饮食同前;模型组继续高脂喂养;消膏转浊组及罗格列酮组改为普通饲料喂养,同时给予相应的药物干预;饮食控制组改为普通饲料喂养。干预12周后,检测大鼠葡萄糖输注率(GIR),肝匀浆中FFA、MDA、SOD的含量,检测肝细胞色素P4502E1的蛋白表达,以及肝脏CYP2E1、胰岛素受体(InsR)和胰岛素受体底物-2(IRS-2)的基因表达。结果:与模型组比较,消膏转浊组可以显著降低大鼠肝组织匀浆中的FFA、MDA含量(P<0.01),同时提高SOD活性(P<0.01);消膏转浊组与罗格列酮组均能够显著提高肥胖型非酒精性脂肪肝大鼠的GIR(P<0.01);消膏转浊组肝细胞中CYP2E1染色阳性的细胞面积和MOD值、CYP2E1 mRNA的表达均显著低于模型组(P<0.01);同时,消膏转浊组与罗格列酮组InsR mRNAI、RS-2 mRNA的表达均显著高于模型组(P<0.01)。结论:高脂饮食可以引起大鼠肝脏脂质过氧化反应以及胰岛素抵抗状态,消膏转浊方可减少肝脏脂质过氧化反应以保护肝脏;同时消膏转浊方通过增加肝脏组织InsR以及IRS-2的表达来改善胰岛素抵抗状态。
Objective: To observe the effect of Xiaoganzhuanzhuo recipe on lipid peroxidation and insulin resistance (IR) in fat-fed rats. Methods: Wistar male rats were randomly divided into normal group, model group, Xiaogan Zhuanzhuo group, rosiglitazone group and diet control group. The normal group was fed the standard diet, and the remaining group of rats fed the high-fat diet. After 8 weeks, the normal group had the same diet; the model group continued to be fed with high-fat diet; the ointment-Zhuanzhuo group and the rosiglitazone group were changed to ordinary feed while the corresponding drug intervention was given; and the diet control group was changed to normal feed. After intervention for 12 weeks, the glucose infusion rate (GIR), FFA, MDA and SOD in the liver homogenate were measured to detect the protein expression of liver cytochrome P4502E1 and the expressions of CYP2E1, InsR and insulin Substrate Substrate-2 (IRS-2) Gene Expression. Results: Compared with the model group, the Xiaofuzhuanzhuo group could significantly reduce the content of FFA and MDA in the rat liver homogenate (P <0.01) and increase the activity of SOD (P <0.01) Glitazone group can significantly improve the GIR of obese non-alcoholic fatty liver rats (P <0.01); CYP2E1-positive cell area and MOD values, CYP2E1 mRNA expression in the group (P <0.01). Meanwhile, the expression of InsR mRNA and RS-2 mRNA in Xiaoguzhuanzhu group and rosiglitazone group were significantly higher than those in model group (P <0.01). Conclusion: High-fat diet can induce lipid peroxidation and insulin resistance in liver of rats, and relieving liver-lipid peroxidation can be reduced by eliminating the ointment to transfer turbidity. At the same time, -2 expression to improve insulin resistance status.