【摘 要】
：
从UniGene库中选取编号为BG231197,来自人鼻咽组织的EST序列.利用Blast检索GenBank的nr数据库和EST数据库,构建EST重叠群.利用人类基因组草图搜索法从成人正常鼻咽组织中PCR
【基金项目】
：
国家重点基础研究发展计划(973计划);
论文部分内容阅读
从UniGene库中选取编号为BG231197,来自人鼻咽组织的EST序列.利用Blast检索GenBank的nr数据库和EST数据库,构建EST重叠群.利用人类基因组草图搜索法从成人正常鼻咽组织中PCR扩增获得该基因全长cDNA,命名为NAPl,GenBank登录号为AYl90326.NAPl基因cDNA序列全长为573 bp,编码由85个氨基酸组成,相对分子质量为9 700的多肽.用α-32P-dCTP标记NAPl基因片段,与含15种正常成人组织的多组织RNA印迹膜杂交,结果表明NAPl基因在淋巴结和气管中高表达,转录本大小约为0.6kb,在其他组织中不表达.NAPl蛋白质与滤泡树突状细胞(follicular dendritic cell,FDC)的一种分泌肽前体(FDC-SP)(AF435080)同源,与其他已知蛋白质无明显同源性.NAP1基因定位在染色体4q13,基因组跨越9 179 bp,含5个外显子和4个内含子.采用差异RT-PCR检测了40例经病理诊断为低分化鳞状上皮癌的鼻咽活检组织及其对侧相应部位的正常鼻咽组织中该基因的表达差异.在40例鼻咽癌中,NAP1基因表达下调的有17例(42.5%),表达上调的有6例(15%),无明显表达差异的有17例(42.5%).原位杂交和免疫组化结果显示该基因在正常鼻咽和鼻咽癌组织间质中的树突状细胞中表达,在其他间质细胞和鼻咽上皮中均不表达.以上结果表明,NAP1为树突状细胞的一种新的多肽,该基因在鼻咽癌组织中表达下调的原因,及其与鼻咽癌发生、发展的关系值得进一步探讨.
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