【摘 要】
：
粒酶B(granzyme B,GrB)是一种重要的丝氨酸蛋白酶参与细胞毒性T淋巴细胞(CTL)和自然杀伤细胞(NK)介导的细胞杀伤过程.为研究粒酶B在肿瘤细胞中异位表达后能否诱导细胞死亡,将
【机 构】
：
第四军医大学生物化学与分子生物学教研室,清华大学生物科学与技术系,第四军医大学人体解剖学教研室
【基金项目】
：
国家高技术研究发展计划(863计划);军队医药卫生科研项目;国家自然科学基金
论文部分内容阅读
粒酶B(granzyme B,GrB)是一种重要的丝氨酸蛋白酶参与细胞毒性T淋巴细胞(CTL)和自然杀伤细胞(NK)介导的细胞杀伤过程.为研究粒酶B在肿瘤细胞中异位表达后能否诱导细胞死亡,将构建的活性型粒酶B(GrBa)基因及其酶活性中心突变型(mGrBa)基因的真核表达载体,以脂质体法瞬时转染HeLa细胞,通过绿色荧光蛋白(GFP)共表达、间接免疫荧光、细胞计数、MTT等方法,观察到GrBa蛋白的异位表达引起多核巨细胞形态异常,并且表达细胞的生长受到抑制.Percoll分离多核巨细胞后,观察到其生长状态较差,是导致生长抑制的直接原因.细胞骨架破坏和具有多极纺锤体的异常有丝分裂,推测是多核巨细胞不断产生的根源.上述结果为GrBa应用于肿瘤基因治疗提供了一定依据.
其他文献
文章分析了服务质量的概念界定、理论流派以及评价模式,之后从公共服务质量评价、公众满意度评价、政府网站质量评价等方面对现有研究进行了分析和论述,从而提出电子政务服务
从生态休闲体育旅游发展的战略观点出发，基于生态休闲体育旅游的发展，阐述生态休闲体育旅游概念和特点，提出生态休闲体育旅游可持续发展机制，旨在促进生态旅游的持续健康发展。
WRKYgenes encode transcription factors that are involved in the regulation of various biological processes. These zinc-finger proteins, especially those members
Hydroxyl radical (·OH)-initiated photooxidation reaction of toluene was carried out in a self-made smog chamber. Four individual seed aerosols such as ammonium
The process of flowering is controlled by a hierarchy of floral genes that act as flowering time genes, inflorescence/floral meristem identity genes, and/or flo
In the past decade, significant knowledge has accumulated regarding gibberellin (GA) signal transduction in rice as a result of studies using multiple approache
Pollination involves a series of complex cellular interactions and signal transduction events. Numerous reports have suggested a central role for protein kinase
Gene silencing can occur either at the transcriptional level or post-transcriptional level or both. Many instances of sequence-specific silencing requires small
To systematically estimate the gene duplication events in closely related species, we have to use comparative genomic approaches, either through genomic sequenc
Abiotic stress is the main factor negatively affecting crop growth and productivity worldwide. The advances in physiology, genetics, and molecular biology have