论文部分内容阅读
目的了解南昌昌北国际机场中华按蚊的氟氯氰菊酯抗药性,初步探索其产生机制。方法应用WHO成蚊生测法检测当地中华按蚊抗药性水平,将生测过程中即将死亡蚊虫归为敏感蚊,存活蚊虫归为抗性蚊,检测并比较两组蚊虫P450单加氧酶、谷胱甘肽巯基转移酶(GST)活性;同时记录每只敏感蚊生测死亡时间,对生测死亡时间与P450单加氧酶、GST活性进行相关分析。结果当地中华按蚊生测死亡率为59.5%;抗性蚊、敏感蚊、实验室敏感蚊的P450单加氧酶活性差异具有统计学意义(F=151.89,P<0.01),其中抗性蚊>敏感蚊>实验室敏感蚊;但三者GST活性差异无统计学意义(F=0.72,P>0.05);蚊虫生测死亡时间与P450单加氧酶活性呈正相关性,相关系数为0.88,回归方程为y=79.479+1.512x;生测死亡时间与GST活性不具有相关性。结论南昌昌北国际机场中华按蚊对氟氯氰菊酯已产生抗药性,P450单加氧酶活性增强可能是其抗药性产生的机制之一。
Objective To understand the resistance of cyfluthrin of Anopheles sinensis in Nanchang Changbei International Airport and explore its mechanism. Methods The WHO anti-mosquito test was used to detect the resistance level of Anopheles sinensis. The dead mosquitoes were classified as sensitive mosquitoes during the life test. The surviving mosquitoes were classified as resistant mosquitoes. The levels of P450 monooxygenase, Glutathione S-transferase (GST) activity was measured. The time of death was measured for each sensitive mosquito, and the correlation between P450 monooxygenase and GST activity was analyzed. Results The local mortality of Anopheles sinensis was 59.5%. The differences of P450 monooxygenase activity between resistant mosquito, sensitive mosquito and laboratory sensitive mosquito were statistically significant (F = 151.89, P <0.01) > Sensitive mosquitoes> sensitive mosquitoes in the laboratory. However, there was no significant difference in the GST activities between the two groups (F = 0.72, P> 0.05). The dead time of mosquitoes was positively correlated with the activity of P450 monooxygenase The regression equation was y = 79.479 + 1.512x; there was no correlation between the measured time of death and GST activity. Conclusion Nanfang Changbei International Airport has developed resistance to cyfluthrin, and the increase of P450 monooxygenase activity may be one of the mechanisms of drug resistance.