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A comprehensive field experiment was made with the support of the project of China Radiometric Calibration Site (CRCS) during June-July 1999. Ground reflectance spectra were measured at Dunhuang Calibration Test Site in the experiment. More than two thousands of spectral curves were acquired in a 20 km × 20 km area. The spectral coverage is from 350 nm to 2500 nm. The measurement values show that reflectance is between 10% and 33% at the VISSWIR spectral region. The standard deviation of reflectance is between 1.0% and 2.0% for the spectral range. Optical characteristics and ground reflectance measurements at the Dunhuang test site, result analysis and error source were described. In addition, a comparison of the reflectance obtained in 1999 with those measured in 1994 and 1996 was also made.