用昆明种小鼠按Wasley’s法快速获取腹腔巨噬细胞。以贴壁法提纯(9～12℃),体外培养的细胞呈单层生长,可存活2～3周。对提纯的细胞用形态学、细胞化学、吞噬及抗胰蛋白酶消化试验等法鉴定。结果:体外培养的细胞具有巨噬细胞的典型特征,属高纯度的巨噬细胞,纯度达97%以上。使用高纯度的细胞必将提高实验研究的质量。 Peritoneal macrophages were rapidly obtained by using Wasley’s method in Kunming mice. Purification by adherent method (9 ~ 12 ℃), cultured in vitro monolayer growth can survive for 2 to 3 weeks. The purified cells were identified by morphology, cytochemistry, phagocytosis and trypsin digestion assay. Results: The cells cultured in vitro have typical characteristics of macrophages. They are macrophages with high purity of over 97%. The use of high-purity cells will surely improve the quality of experimental research.