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1983-1984年,对北京、浙江、山东等地啤酒花种植地区的啤酒花冠瘿病发病情况进行了调查,并采集、分离到啤酒花根癌土壤杆菌16株,对其生物型、质粒类型、寄主范围和对土壤杆菌素84(agrocin 84)的敏感性进行了测定。证明所测菌株都属于土壤杆菌生物Ⅰ型菌,质粒类型为胭脂碱型(nopaline),具有较广的寄主范围,其中11株菌对土壤杆菌素84敏感,5株菌不敏感。对10株菌的质粒进行了检测。在琼脂糖凝胶电泳图上表明,所有菌株都含一个与pTiC58大小相同的质粒,此外,大多数菌株还含有1-3个隐蔽质粒。
In 1983-1984, the incidence of corns galls disease in hops planting areas of Beijing, Zhejiang and Shandong was surveyed and 16 strains of Agrobacterium tumefaciens from hops were collected and analyzed. The biological types, the type of plasmid, the host range And the sensitivity to agrocin 84. The results showed that the tested strains belonged to Agrobacterium tumefaciens type Ⅰ. The plasmid type was nopaline and had a wide host range. Eleven strains were sensitive to streptozotocin 84 and five strains were insensitive. Ten strains of plasmids were tested. The agarose gel electrophoresis showed that all strains contained one plasmid of the same size as pTiC58, and most of the strains also contained one to three covert plasmids.