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A computer-assisted method is presented for simultaneous optimization of pH and ion con-centration selection for the optimal separation in reversed-phase HPLC.The method is based on apolynomial estimation from nine preliminary experiments according to two-factor rectangular design.This is followed by a two-dimension computer scanning technique.Resolution is used as the selectioncriterion.Good agreement was obtained between predicted data and experimental results.
A computer-assisted method is presented for simultaneous optimization of pH and ion con-centration selection for the optimal separation in reversed phase HPLC. The method is based on apolynomial estimation from nine preliminary experiments according to two-factor rectangular design. This is followed by a two-dimension computer scanning technique. Resolution is used as the selection criterion. Good agreement was obtained between predicted data and experimental results.