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目的:探讨Ad bFGF、Ad SOX9、Ad CTGF和AdTIMP1基因对体内退变髓核细胞的影响或作用。方法:选择成年新西兰大白兔120只,使用纤维穿刺法进行动物模型手术,注入重组腺病毒转染bFGF、SOX-9、CTGF及TIMP-1,术后行X片、RT-PCR检测以及II型胶原检测,结果进行统计学对比。结果:X片检测中椎间盘处理方式对椎间盘高度的改变经统计学分析有统计学意义(P<0.05),对各节段之间的差异进行方差分析,结果显示对照组和生理盐水组之间的差异经统计学分析有统计学意义(P<0.05),转染组和生理盐水组之间的差异经统计学分析有统计学意义(P<0.05)。在注射重组腺病毒转染bFGF、SOX-9、CTGF和TIMP-1载体后,bFGF、SOX-9、CTGF及TIMP-1和II型胶原mRNA的表达水平均有明显提高(P<0.05),对照组中bFGF和II型胶原mRNA水平表现为不同程度的降低。在重组腺病毒转染bF-GF、SOX-9、CTGF以及TIMP-1中的II型胶原的表达检测中,3组的均数方差齐性,差异经统计学分析有统计学意义(P<0.05),再将3组数据行两两比较,转染组与对照组、对照组与生理盐水组,均数的差异经统计学分析有统计学意义(P<0.05)。结论:bFGF、SOX9、CTGF和TIMP-1基因可以在退变髓核细胞或组织中持续大量表达,这些基因可能均参与到椎间盘退变的发病机制中。
Objective: To investigate the effect of Ad bFGF, Ad SOX9, Ad CTGF and AdTIMP1 on degenerative nucleus pulposus cells in vivo. Methods: One hundred and twenty New Zealand white rabbits were selected and operated on by animal model. The recombinant adenovirus was used to transfect bFGF, SOX-9, CTGF and TIMP-1. X-ray, RT- Collagen detection, the results were statistically compared. Results: The change of intervertebral disc height in X-ray examination was statistically significant (P <0.05). The analysis of variance was used to analyze the differences among the three groups. The results showed that between the control group and the normal saline group (P <0.05). The difference between the transfection group and the saline group was statistically significant (P <0.05). The mRNA expression levels of bFGF, SOX-9, CTGF, TIMP-1 and type II collagen in bFGF, SOX-9, CTGF and TIMP-1 were significantly increased after injection of recombinant adenovirus (P <0.05) The control group bFGF and type II collagen mRNA levels showed varying degrees of reduction. In the expression of type II collagen in bF-GF, SOX-9, CTGF and TIMP-1 transfected with recombinant adenovirus, the homogeneity of mean of the three groups was statistically significant (P < 0.05). The data of three groups were compared with each other. The difference of mean between the transfection group and the control group, the control group and the saline group was statistically significant (P <0.05). CONCLUSION: The bFGF, SOX9, CTGF and TIMP-1 genes can be expressed continuously in degenerative nucleus pulposus cells or tissues. These genes may participate in the pathogenesis of disc degeneration.