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目的建立人胃癌裸鼠移植瘤模型,观察血管紧张素转换酶抑制剂(ACEI)对胃癌生长和淋巴管生成的影响。方法设立对照组、培哚普利组、卡托普利组,定期观察各组肿瘤生长情况测量肿瘤体积,3周后取出肿瘤标本,采用免疫组织化学测定各组的基质金属蛋白酶(MMP)-7、血管内皮生长因子(VEGF)-C的表达和癌周微淋巴管密度。结果培哚普利组、卡托普利组移植瘤体积与对照组比较明显受到抑制,差异有统计学意义(P<0.01),培哚普利组、卡托普利组肿瘤组织中的MMP-7、VEGF-C(P<0.05)和LMVD与对照组比较均显著降低,差异有统计学意义(P<0.01)。结论卡托普利和培哚普利能明显抑制人胃癌裸鼠移植瘤的生长以及新生淋巴管的形成。
Objective To establish a human gastric cancer xenograft model in nude mice and observe the effect of angiotensin converting enzyme inhibitor (ACEI) on gastric cancer growth and lymphangiogenesis. Methods The control group, perindopril group and captopril group were established. The tumor volume was observed regularly and the tumor volume was measured. The tumor samples were taken out after 3 weeks. The levels of matrix metalloproteinase (MMP) - 7, vascular endothelial growth factor (VEGF) -C expression and pericancerous lymphatic vessel density. Results The volume of transplanted tumor in perindopril group and captopril group was significantly lower than that in control group (P <0.01). MMPs in perindopril group and captopril group were significantly lower than those in control group -7, VEGF-C (P <0.05) and LMVD were significantly decreased compared with the control group, the difference was statistically significant (P <0.01). Conclusion Captopril and perindopril can significantly inhibit the growth of human gastric cancer xenografts and the formation of lymphangiogenesis.