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目的:观察诺氟沙星对EEG的影响和对脑组织超微结构的毒性作用。方法:大鼠右侧脑室注射诺氟沙星150和300μg·kg~(-1)后,于皮层感觉运动区记录EEG并观察脑组织超微结构的变化。结果:大鼠EEG均出现痫样放电,两组大鼠右侧和左侧棘波发生时间为168±129,51±35s和276±138,118±65s。以注射侧较早发生,波形多变,幅度和频率逐渐增大,并伴肢体抽搐的行为学改变。电镜下,300μg·kg~(-1)组大鼠双侧大脑皮层、海马及小脑均有明显的神经元变性,包括高尔基复合体、粗面内质网及神经毡的髓样变性、细胞质肿胀、细胞核及染色质溶解、胶质细胞坏死等,并出现亮神经元和暗神经元改变,以暗神经元为著。结论:诺氟沙星点燃癫痫后,大鼠脑组织超微结构受到广泛损伤。
Objective: To observe the effects of norfloxacin on EEG and its toxic effects on the ultrastructure of brain tissue. Methods: The rats were injected Norfloxacin 150 and 300 μg · kg ~ (-1) into the right lateral ventricle. EEG was recorded in the sensory zone of cortex and the ultrastructure of brain was observed. Results: The epileptiform discharge occurred in both EEG rats. The right and left spikes occurred in 168 ± 129, 51 ± 35s and 276 ± 138, 118 ± 65s in both groups. In the injection side occurred earlier, the waveform changes, the amplitude and frequency gradually increased, and with behavioral changes in limb seizures. Under the electron microscope, obvious neuronal degeneration was found in bilateral cerebral cortex, hippocampus and cerebellum of 300μg · kg ~ (-1) group, including myeloid degeneration of Golgi complex, rough endoplasmic reticulum and neuropil, cytoplasm swelling , Nuclear and chromatin dissolution, glial cell necrosis, etc., and bright neurons and dark neurons change to dark neurons as a. Conclusion: Norfloxacin ignites epilepsy, the ultrastructure of rat brain tissue is extensively damaged.