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目的 :探讨本芴醇的抗疟作用机制。方法 :采用流式细胞术 (FCM)分析了本芴醇和氯喹对鼠伯氏疟原虫K173株DNA含量及溶酶体pH值的影响。结果 :对照组疟原虫DNA含量在各时间点没有显著变化。本芴醇单次给药后 ,随时间推移疟原虫DNA含量逐渐减少 ,药后 2h两给药组疟原虫红内期DNA含量开始降低 ,到 16h降到最低 ,但药后 2 4hDNA含量又有所回升。本芴醇单次给药后 1h起疟原虫溶酶体pH值开始升高 ,药后 3h升至最高 ,药后 4h原虫溶酶体pH值恢复至药前水平。对照药氯喹对疟原虫DNA含量和溶酶体pH值也有相同影响。结论 :本芴醇的抗疟作用与其抑制DNA合成相关 ,但与其升高溶酶体pH值的关系不明确
Objective: To investigate the anti-malarial mechanism of benflumetol. Methods: The effects of benflumetol and chloroquine on DNA content and lysosomal pH of P. berghei K173 strain were analyzed by flow cytometry (FCM). Results: DNA content of the control group did not change significantly at all time points. After a single administration of lumefantrine, the DNA content of Plasmodium decreased gradually with the passage of time. DNA content of Plasmodium started to decrease within 2 h after drug administration and was lowest at 16 h after drug administration. However, The rise. The lyphotobacterial lysosomal pH began to increase from 1 hour after the single administration of lumefantrine, and reached the peak at 3 hours after drug administration. The protozoal lysosomal pH returned to the pre-drug level 4 hours after the drug administration. The control drug chloroquine also had the same effect on the DNA content of plasmodium and lysosomal pH. Conclusion: The antimalarial activity of lumefantrine is related to its inhibitory effect on DNA synthesis, but its relationship with increasing lysosomal pH value is not clear