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目的通过微透析-高效液相神经递质分析技术观察丘脑底核(subthalamic nucleus,STN)脑深部电刺激(deep brain stimulation,DBS)对偏侧帕金森(parkinson’s disease,PD)模型猴苍白球内侧部(globus pallidusinternus,GPi)氨基酸类神经递质水平的影响。探讨PD的发病机制及DBS治疗PD的可能作用机制。方法成功制备的偏侧PD模型猴4只,微透析方法收集未注药侧GPi的细胞外液以及注药侧STN高频电刺激(highfrequncy stimulation,HFS)过程中以及刺激前后同侧GPi的细胞外液,应用高效液相-荧光法分析谷氨酸(gluta-mate,Glu)和γ-氨基丁酸(gamma-aminobutyric acid,GABA)的水平变化。结果 MPTP注药侧GPi细胞外液中Glu含量较未注药侧升高,而GABA含量却下降。高频电刺激偏侧PD猴模型的STN后,同侧的GPi细胞外液中Glu的含量明显上升,而GABA的含量无明显变化。结论无法用单一的神经元抑制机制解释STN-HFS的作用机制,STN-HFS的作用不只是局限于STN核团本身,对整个基底节环路都有影响。
Objective To investigate the effect of deep brain stimulation (DBS) by subthalamic nucleus (STN) on the medial side of the monkey globus pallidus (Parkinson’s disease, PD) model by microdialysis - HPLC technique. (Globus pallidus internus, GPi) amino acid neurotransmitter levels. To investigate the pathogenesis of PD and the possible mechanism of DBS in the treatment of PD. Methods Four hemiparkinsonian PD models were successfully prepared. The microdialysis method was used to collect the extracellular fluid of uninjected GPI and the high-frequency STF high-frequency stimulation (HFS) on the uninjected side and the cells of ipsilateral GPi before and after stimulation In vitro, the levels of glutamate (Glu) and gamma-aminobutyric acid (GABA) were analyzed by HPLC-fluorescence method. Results Compared with the untreated side, the content of Glu in GPi extracellular fluid of MPTP injection side increased, but the content of GABA decreased. High-frequency electrical stimulation of hemiparkinsonian model of STN after STN, ipsilateral GPi extracellular fluid Glu increased significantly, while the content of GABA no significant change. Conclusion The mechanism of STN-HFS can not be explained by a single neuron repression mechanism. The role of STN-HFS is not limited to the STN nucleus itself, but affects the entire basal ganglia circuits.