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目的:建立适用于二仙汤中淫羊藿苷和盐酸小檗碱的含量测定方法。方法:采用HPLC法,选用Diamonsil C18色谱柱(200mm×4.6mm,5μm),以乙腈-0.1%磷酸(30∶70)为流动相,检测波长为270nm。结果:淫羊藿苷进样量在0.08624~0.4312μg范围内,线性关系良好(r=0.9998);盐酸小檗碱进样量在0.14~0.7μg范围内,线性关系良好(r=0.9999);淫羊藿苷平均回收率为98.4%(RSD为1.6%,n=6),盐酸小檗碱平均回收率为96.4%(RSD为1.8%,n=6)。结论:HPLC法同时测定淫羊藿苷和盐酸小檗碱方法简单,重复性好,结果准确可靠,可用于二仙汤的含量测定。
Objective: To establish a method for the determination of icariin and berberine hydrochloride in Erxian Decoction. Methods: The HPLC method was used to select Diamonsil C18 column (200mm×4.6mm, 5μm) with acetonitrile-0.1% phosphoric acid (30∶70) as the mobile phase. The detection wavelength was 270nm. Results: In the range of 0.08624-0.4312μg, there was a good linear relationship (r=0.9998). The berberine hydrochloride injection ranged from 0.14 to 0.7μg with good linearity (r=0.9999). The average recovery of icariin was 98.4% (RSD 1.6%, n=6), and the average recovery of berberine hydrochloride was 96.4% (RSD 1.8%, n=6). Conclusion: Simultaneous determination of icariin and berberine hydrochloride by HPLC method is simple, reproducible and accurate and reliable. It can be used for the determination of Erxian decoction.