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目的 了解结核分枝杆菌异烟肼耐药株KatG基因的突变情况 ,建立快速筛选耐药株的方法。方法 通过聚合酶链反应 单链构象多态性 (PCR SSCP)和聚合酶链反应 限制性片段长度多态性 (PCR RFLP)分析法对 6 4株结核菌临床分离株的katG基因的突变进行分析。结果 46株敏感株和结核菌H37Rv标准菌株的katG基因均无SSCP泳动异常和MSPⅠ酶切位点的改变。结论 大多数结核菌耐异烟肼分离株有katG基因的突变 ,而突变位点大多是第 315位密码子AGC→ACC。PCR SS CP和PCR RFLP可能成为快速筛选结核菌耐异烟肼突变株的方法
Objective To understand the mutation of KatG gene of isoniazid-resistant strain of Mycobacterium tuberculosis and establish a rapid screening method of resistant strains. Methods The mutation of katG gene in 64 clinical isolates of Mycobacterium tuberculosis was analyzed by single strand conformation polymorphism (PCR SSCP) and polymerase chain reaction restriction fragment length polymorphism (PCR RFLP) . Results There was no change of SSCP motility and MSP Ⅰ cleavage site in katG gene of 46 sensitive strains and standard strain of H37Rv. Conclusion Most isolates of Mycobacterium tuberculosis isolates of isoniazid have the mutation of katG gene, and most of the mutation sites are AGC → ACC at codon 315. PCR SS CP and PCR RFLP may be the method for rapid screening of M. tuberculosis isoniazid-resistant mutants