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除白念珠菌外,其它念珠菌种所致的感染也在增加.作者研究了人外周血细胞——单核细胞(MO)、中性粒细胞(PMN)和淋巴细胞(PBL)对热带念珠菌的杀伤作用.将取自健康志愿者的外周血用Ficoll-Paque密度梯度离心法分离PBL、培养法分离MO、等渗Percoll梯度法分离PMN.取热带念珠菌临床分离株(LF-139)接种在YEPD液基经23℃振荡培养的扩增中期细胞作靶细胞,?别与不同量的PMN、MO或PBL在24孔培养板中混合,经不同时间培养(37℃、5%CO_2)后进一步检测菌落形成单位(CFU),计算对菌的相对杀伤率[CFU(对照管)-CFU(实验管)/CFU(对照管)×%].同时分别观察重组白细胞介素2(γIL-2)、肿癌坏死因子(TNF)γ或
In addition to C. albicans, infections caused by other Candida species are also increasing. The authors studied the effects of human peripheral blood monocytes (MOs), neutrophils (PMNs) and lymphocytes (PBLs) on Candida tropicalis PBMCs were isolated from peripheral blood of healthy volunteers by Ficoll-Paque density gradient centrifugation, isolated from culture supernatants by Percoll gradient centrifugation, and isolated from Candida tropicalis (LF-139) The target cell was expanded at YEPD liquid medium at 23 ℃ and mixed with different amount of PMN, MO or PBL in 24-well culture plate and cultured at different times (37 ℃, 5% CO 2) The CFU (CFU), CFU (experimental tube) / CFU (control tube) ×%] were further tested for colony forming units (CFU) and the relative killing rates of recombinant IL-2 ), Tumor necrosis factor (TNF) gamma or