论文部分内容阅读
615小棕鼠的H615肝癌(H615)是由我国所建立的一株移植性自发性肝癌模型。在病理形态上是一种分化程度较好的肝细胞癌。小白鼠的HepA腹水肝癌(Hep A)是一种恶性程度较高的肝癌。对这两种肝癌我们测定了丙酮酸激酶(PyK)和醛缩酶(ALD)及其同工酶的活力,观察到(1)在Hep A中PyK及ALD总活力都明显增加,而在H615中仅PyK总活力增加。在两种肝癌中,凡属胎儿型或增殖型的同工酶如K型PyK、A型ALD的比例都较正常肝升高,而属成年型或分化型的同工酶如L型PyK、B型ALD的比例都较正常肝降低,但在H615中的变化不如HepA中的明显。(2)两种肝癌宿主肝的同工酶谱可类似一般肝癌所见到者。提示肝癌细胞可能分泌一类化学物质引起宿主肝中PyK和ALD同工酶谱的变化。
H615 liver cancer (H615) of 615 small brown mouse is a transplantable spontaneous liver cancer model established in China. Pathologically, it is a hepatic carcinoma with a better differentiation. HepA ascites liver cancer (Hep A) is a liver cancer with a high degree of malignancy. We measured the activity of pyruvate kinase (PyK), aldolase (ALD) and its isoenzymes in both hepatocarcinomas and observed that (1) the total activity of PyK and ALD increased significantly in Hep A, whereas in H Only PyK’s total vitality increased. In both liver cancers, the ratio of fetal or proliferative isoenzymes such as K type PyK and type A ALD is higher than that of normal liver, and adult type or differentiated isoenzymes such as L type PyK, The proportion of type B ALD was lower than that of normal liver, but the change in H615 was not as obvious as in HepA. (2) The isoenzyme patterns of the two liver cancer host livers can be similar to those seen in general liver cancer. It suggests that hepatoma cells may secrete a class of chemical substances that cause changes in the isoenzyme patterns of PyK and ALD in the host liver.