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目的：观察柯萨基Ｂ组病毒（ＣｏｘＢ）性心肌炎患儿血清肿瘤坏死因子α（ＴＮＦ－α）、白细胞介素６（ＩＬ－６）、白细胞介素８（ＩＬ－８）和可溶性白细胞介素２受体（ｓＩＬ－２Ｒ）的水平变化，评价ＣｏｘＢ感染时机体细胞因子的应答及其在心肌细胞免疫病理损伤中的意义。方法：检测血清采自２５例病毒性心肌炎患儿，其中１６例血清ＣｏｘＢ抗原和特异性ＩｇＭ抗体检测均阳性（Ｍ１组）；另９例为血清ＣｏｘＢ抗原阴性、特异性ＩｇＭ阳性（Ｍ２组）。１０例正常儿童血清为对照。血清细胞因子含量测定及ＣｏｘＢ抗原、特异性ＩｇＭ抗体检测均采用酶联免疫吸附试验。结果：ＣｏｘＢ病毒性心肌炎患儿血清ＴＮＦ－α为（２９７．４±２６８．０）ｎｇ／Ｌ、ＩＬ－８为（４８０．９±４３５．５）ｎｇ／Ｌ和ＩＬ－６为（２０７．０±２９３．９）ｎｇ／Ｌ，均显著高于正常对照组；然而，ｓＩＬ－２Ｒ为（３９８４．８±１７８１．０）ｎｇ／Ｌ，与正常对照组相比无明显差异。其中Ｍ１组血清ＴＮＦ－α、ＩＬ－６的含量与Ｍ２组相比无显著性差异，但血清ＩＬ－８含量明显高于Ｍ２组。血清ＴＮＦ－α和ＩＬ－８的浓度变化存在明显的正相关。结论：ＣｏｘＢ病毒性心肌炎患儿血清ｓＩＬ－２Ｒ水平基本正常?
Objective: To observe the changes of serum tumor necrosis factor α (TNF-α), interleukin 6 (IL-6), interleukin 8 (IL-8) and soluble interleukin-8 in children with CoxB myocarditis 2 receptor (sIL-2R) level changes, evaluation of CoxB infection in response to cytokines and its role in myocardial cellular immune pathology. Methods: Serum samples were collected from 25 children with viral myocarditis. 16 cases were positive for serum CoxB antigen and specific IgM antibody (M1 group); the other 9 cases were negative for serum CoxB antigen (M2 group) . 10 normal children’s serum as a control. Serum levels of cytokines and CoxB antigen, specific IgM antibodies were detected by enzyme-linked immunosorbent assay. Results: Serum TNF-α was (297.4 ± 268.0) ng / L in children with CoxB viral myocarditis, (480.9 ± 435.5) ng / L in IL-8 and (207) in children with CoxB viral myocarditis. 0 ± 293.9 ng / L, all of which were significantly higher than that of the normal control group. However, sIL-2R was (3984.8 ± 1781.0) ng / L, which was not significantly different from the normal control group. The level of serum TNF-α and IL-6 in group M1 was not significantly different from that in group M2, but the level of serum IL-8 in group M1 was significantly higher than that in group M2. Serum TNF-α and IL-8 concentrations have a significant positive correlation. Conclusion: The level of sIL-2R in children with CoxB viral myocarditis is basically normal.