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稻褐飞虱是水稻的主要虫害之一,培育优良的抗性基因聚合系对于防治稻褐飞虱具有重要的意义。本研究通过回交、分子标记辅助选择和接虫鉴定三者相结合的办法,将抗稻褐飞虱基因Bph3和Bph24(t)分别导入主栽杂交水稻恢复系广恢998、9311、R15、明恢63、R29中,最终获得遗传稳定的Bph3导入系32份和Bph24(t)导入系22份,获得Bph3Bph24(t)优良聚合系13份。人工接虫鉴定结果显示,Bph3和Bph24(t)导入系对稻褐飞虱抗性达到中抗至抗的水平,平均分别为3.96级和3.84级,其中Bph24(t)导入系对稻褐飞虱抗性略强于Bph3导入系;Bph3Bph24(t)聚合系抗性最强,对稻褐飞虱抗性达到2.38~2.65级、平均2.52级的抗级水平,表明抗性基因聚合对稻褐飞虱的抗性具有累加基因效应。SSR标记分析表明抗性基因导入系、聚合系的遗传背景回复率达到90%以上。农艺性状分析显示,所选的多数基因聚合系的株高、剑叶长宽度、有效穗、千粒重等主要农艺性状与受体恢复系差异不显著。证明本研究已成功获得5个主栽杂交水稻恢复系的抗稻褐飞虱基因导入系和聚合系,这些抗性改良恢复系具有良好应用前景,并且为建立完善的水稻抗性育种平台提供了重要育种材料基础。
Nilaparvata lugens (Nilaparvata lugens) is one of the major pests in rice. It is of great significance to cultivate excellent resistance gene polymerases to control Nilaparvata lugens (Nilaparvata lugens). In this study, Bph3 and Bph24 (t) genes were introduced into the restorer lines of main hybrid rice Guanghui 998, 9311, R15, Minghui and Minghui by combining backcross, molecular marker-assisted selection 63 and R29, 32 genetically stable Bph3 and 22 Bph24 (t) transformants were finally obtained, and 13 excellent polymerization systems of Bph3Bph24 (t) were obtained. The results of artificial insecticidal identification showed that the resistance of Bph3 and Bph24 (t) lines to Nilaparvata lugens (Nilaparvata lugens) was at 3.96 and 3.84, respectively, of which Bph24 (t) Bph3Bph24 (t) was the strongest and the resistance to the brown planthopper (BPH) was 2.38-2.65, with an average of 2.52 resistance level, indicating that the resistant gene polymerization had additive genes for the resistance of the brown planthopper effect. SSR marker analysis showed that the resistance gene was introduced into the lineage, and the genetic background of the polymerized lines was over 90%. Agronomic traits analysis showed that the major agronomic traits and receptor restorer lines of most of the selected lines were not significantly different in plant height, flag leaf width, effective panicle, 1000-grain weight. It is proved that this study has successfully obtained the introduced lines and the polymerization lines of resistance to brown planthopper in five major restorer lines of hybrid rice. These improved lines have good application prospects and provide important breeding for the establishment of a perfect rice breeding platform Material basis.