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观察缺氧及再给氧对体外培养星形胶质细胞存活能力及谷氨酸摄取功能的影响。方法:取生后1～2d新生昆明小鼠大脑皮层进行星形胶质细胞原代培养,培养 1周左右予以缺氧。缺氧时间分别为 12、24、48 h,并取缺氧24 h后再给氧0、12、24、48 h的细胞观察其形态、死亡细胞数目、乳酸脱氢酶活性及谷氨酸摄取能力的变化。结果:缺氧组及再给氧组星形胶质细胞死亡数较对照组无明显变化,缺氧前、后乳酸脱氢酶活性亦无明显改变,缺氧组谷氨酸摄取功能较对照组下降30%～60%,并随缺氧时间延长而明显下降(与对照组相比,P<0.01),再给氧24 h内谷氨酸摄取能力有所恢复,但未达正常水平。结论:星形胶质细胞与神经元相比,对缺氧培养液较为耐受,缺氧对星形胶质细胞存活能力无明显影响,但细胞摄取谷氨酸能力有所改变。
To observe the effects of hypoxia and reoxygenation on viability of astrocytes and glutamate uptake in vitro. Methods: Primary cultured astrocytes were cultured in neonatal Kunming mouse cortex 1 ~ 2 days after birth and hypoxia was cultured for about 1 week. Hypoxia time were 12,24,48 h, and hypoxia for 24 h and then oxygen 0,12,24,48 h cells were observed morphology, the number of dead cells, lactate dehydrogenase activity and glutamate uptake Change in ability. Results: The number of astrocyte deaths in hypoxia group and reoxygenation group had no significant change as compared with the control group, and there was no significant change in lactate dehydrogenase activity before and after hypoxia. The function of glutamate uptake in hypoxia group was higher than that in control group Decreased by 30% -60%, and decreased significantly with the prolongation of hypoxia (compared with the control group, P <0.01). However, the glutamic acid uptake capacity recovered within 24 hours of oxygenation but did not reach the normal level. CONCLUSION: Compared with neurons, astrocytes are more tolerant to hypoxia and the hypoxia has no effect on the viability of astrocytes. However, the ability of astrocytes to uptake glutamate changes.