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目的 分离纯化正常大鼠脾脏 2 0S蛋白酶体 ,并观察其形态特征。方法 采用分级盐析、离子交换层析及凝胶过滤方法 ,分离纯化了正常大鼠脾脏 2 0S蛋白酶体 ,并进行透射电镜观察。结果 所制备的2 0S蛋白酶体纯度较高 ,非变性聚丙烯酰胺凝胶电泳 (PAGE)显示 1条带 ,SDS 聚丙烯酰胺凝胶电泳 (SDS PAGE)显示 8条带 ,分子量为 ( 19.8～ 31.7)× 10 3;电镜观察显示 ,脾脏 2 0S蛋白酶体呈现典型的圆筒状 ,具有同其它细胞 2 0S蛋白酶体一样的形态特征。结论 这为进一研究 2 0S蛋白酶体结构与功能的关系奠定了基础。
Objective To isolate and purify 20S proteasome of normal rat spleen and observe its morphological characteristics. Methods The splashed 20S proteasomes of normal rats were isolated and purified by fractional salting-out, ion exchange chromatography and gel filtration. Transmission electron microscopy was performed. Results The 20S proteasome was highly purified. PAGE showed 1 band and SDS PAGE showed 8 bands with the molecular weight of (19.8 ~ 31.7 ) × 10 3; electron microscopy showed that the spleen 20S proteasome showed a typical cylindrical shape, with other cells 20S proteasome the same morphological characteristics. Conclusion This laid the foundation for the further study on the relationship between the structure and function of the 20S proteasome.