论文部分内容阅读
目的建立大鼠同种异体原位气管移植模拟肺移植后发生的闭塞性细支气管炎(obliterative bronchiolitis,OB)的病变过程,为研究OB提供动物模型。方法选用近交系Brown Norway(BN)和Lewis大鼠进行原位气管移植,同系移植组:Lewis(供体)→Lewis(受体),18只;异系移植组:BN(供体)→Lewis(受体),18只。于移植后7、14、30、60 d通过Micro-CT扫描观察移植气管影像学变化,并评价阻塞情况;7、30、60 d取出移植气管,HE染色观察移植物的组织病理变化。结果各时间点,同系移植组移植气管内膜均无明显增厚,炎症细胞浸润少,气道阻塞率在8%左右;异系移植组移植气管内膜明显增厚,早期有大量中性粒细胞、淋巴细胞浸润,晚期主要是淋巴细胞浸润伴纤维组织增生,气道明显狭窄、阻塞,其中以第7天阻塞最重,随后渐改善,7、14、30、60 d气道阻塞率分别为(56.8±9.04)%、(51.05±9.79)%、(44.01±7.92)%和(42.97±8.82)%,各时间点异系移植组和同系移植组比较差异有统计学意义(P<0.01)。结论原位气管移植能够稳定形成可模拟肺移植后OB的气道阻塞性病变,克服了肺移植和异位气管移植用于OB研究的一些限制,可更好地作为OB的动物模型应用于实验研究中。
Objective To establish a rat model of allogeneic tracheal transplantation in order to simulate the pathological process of obliterative bronchiolitis (OB) after lung transplantation and to provide an animal model for the study of OB. METHODS: In situ tracheal transplantation was performed using inbred lines Brown Norway (BN) and Lewis rats. In the syngeneic transplantation group, Lewis (donor) → Lewis (receptor), 18; allograft group: BN Lewis (acceptor), 18. At 7, 14, 30 and 60 days after transplantation, the tracheal changes of the trachea were observed by Micro-CT scanning and the obstruction was evaluated. The grafts were removed at 7, 30 and 60 days after operation, and the histopathological changes were observed by HE staining. Results At all time points, there was no significant thickening of tracheal intima in allograft transplantation group with less infiltration of inflammatory cells, and the rate of airway obstruction was about 8%. In tracheal transplantation group, the tracheal intima was thickened obviously and a large number of neutrophils The lymphocytes were infiltrated by lymphocytes and lymphocytes. The advanced lymphocytes were infiltrated with fibrosis, the airways were stenosed and obstructed. The most obstructive airways were blocked on the 7th day, and then gradually improved. The airway obstruction rates at 7, 14, 30 and 60 days (56.8 ± 9.04)%, (51.05 ± 9.79)%, (44.01 ± 7.92)% and (42.97 ± 8.82)%, respectively, there was significant difference between allograft transplantation group and homograft group ). CONCLUSION: In situ tracheal transplantation can stably form obstructive airway obstruction that obstructs OB after lung transplantation and overcomes the limitations of lung transplantation and ectopic tracheal transplantation for OB studies and may be better applied as an animal model of OB in the experiment researching.