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为了全面评价冷贮对米蛾Corcyra cephalonica Stainton卵繁育螟黄赤眼蜂Trichogramma chilonis Ishii的影响,本试验采用米蛾卵为寄主,以冷贮温度(3和10℃)、冷贮虫龄(以接蜂开始时间为起点,接蜂后1,2,3,4,5,6,7,8和9日龄)和冷贮时间(1,2,3和4周)为试验因子,研究了冷贮对螟黄赤眼蜂活蛹率、羽化率和畸形率的影响。结果表明:3个因素均能单独或互作显著影响活蛹率、羽化率和畸形率(P<0.05)。综合各冷贮虫龄对低温的反应,螟黄赤眼蜂对3℃较敏感,此温度下1-3日龄的活蛹率和各虫龄的羽化率均显著下降(P<0.05),不适宜长期冷贮;10℃下各虫龄的活蛹率受冷贮时间的影响不显著,1-3日龄的羽化率受冷贮时间的影响也不显著,冷贮4周后仍有74%以上的羽化率,10℃适宜冷贮。不同冷贮温度下冷贮适宜虫龄不同。在10℃下,最适冷贮虫龄是3日龄的赤眼蜂,此虫龄不同冷贮时间的活蛹率(74.09%~77.59%)和羽化率(74.26%~90.37%)均与对照无显著差异(P>0.05),但显著高于其他虫龄(P<0.05)。此虫龄在10℃下冷贮1~3周,畸形率在27%以内。结果表明米蛾卵内螟黄赤眼蜂不同冷贮温度下适宜冷贮虫龄不同。
In order to comprehensively evaluate the effect of cold storage on the Trichogramma chilonis Ishii, a dominant species of Corcyra cephalonica Stainton, the cold storage temperature (3 and 10 ℃) As the starting point, then 1, 2, 3, 4, 5, 6, 7, 8 and 9 days after beekeeping) and cold storage time (1, 2, 3 and 4 weeks) Effect of cold storage on live pupal rate, emergence rate and deformity rate of Trichogramma. The results showed that all three factors could affect the pupal rate, emergence rate and deformity rate significantly (P <0.05). Based on the responses of cold-shelled instars to low temperature, Trichogramma ostriniae was more sensitive to 3 ℃, and the live pupae rate and the emergence rate of each instar larvae decreased significantly at this temperature (P <0.05) Not suitable for long-term cold storage; the survival rate of pupae at each instar larvae at 10 ℃ was not significantly affected by cold storage time, and the emergence rate at 1-3 days was not significantly affected by cold storage time. Emergence rate above 74%, 10 ℃ suitable cold storage. Different cold storage temperature appropriate storage age cold storage different. At 10 ℃, the optimum storage temperature was 3 days old Trichogramma, the live pupae rate (74.09% ~ 77.59%) and emergence rate (74.26% ~ 90.37%) at different cold storage time were significantly correlated with No significant difference (P> 0.05), but significantly higher than other worm age (P <0.05). The insect age at 10 ℃ cold storage 1 to 3 weeks, deformity rate of 27%. The results show that the rice moth oviparous yellow brown eye bee under different cold storage temperature suitable cold storage insects different ages.