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母胎耐受机制的阐明,将为器官移植免疫耐受方案的研究提供重要启示.本研究旨在阐明妊娠状态对父系来源移植皮片的存活是否有保护作用.2月龄雌性C57BL/6小鼠和2～4月龄BALB/c雄性小鼠同笼受孕.采用流式细胞技术确定妊娠过程中调节性T细胞(Treg)比例的时间变化规律.以单向混合淋巴细胞反应(MLR)手段比较研究妊娠对于父系来源脾细胞刺激后产生的增殖反应的影响.通过同种异体小鼠全厚皮片移植模型,观察妊娠对于父系来源移植皮片的存活是否具有保护作用.并用分子生物学技术研究此种效能的可能机制.结果显示,C57BL/6小鼠妊娠过程中,Treg占CD4+T细胞的比例从妊娠前的4.2%逐渐上升,受孕8天左右达到高峰值(6.8%),此后开始下降并逐渐回复至基线水平.MLR结果表明,针对父系来源脾细胞的刺激,妊娠组较对照组呈现显著的低反应性,其平均刺激指数分别是7.8和13.6(P<0.05).定量PCR研究表明,血红素加氧酶-1和吲哚胺2,3双加氧酶mRNA在胎盘高表达,在脾脏低表达(P<0.05).父系来源的移植皮片的平均存活时间在妊娠组和非妊娠组分别是7.67和7.08天,无统计学差异(P>0.05).由此认为,在小鼠妊娠过程中,尽管出现了具有免疫抑制功能的Treg的比例增加,尽管有针对父系来源刺激细胞的较低的MLR反应性,但是单次妊娠对于父系来源的移植皮片的存活,在本研究条件下,未能显示具有统计学意义的保护作用.
The elucidation of the mechanism of maternal-fetal tolerance will provide important implications for the study of the immune tolerance of organ transplantation.This study aimed to clarify whether the pregnancy status had protective effects on the survival of the grafted parental grafts.2-month-old female C57BL / 6 mice And 2 to 4 months old BALB / c male mice were caged.Methods Flow cytometry was used to determine the time course of the proportion of regulatory T cells (Tregs) during pregnancy.According to the one-way mixed lymphocyte reaction (MLR) To study the effect of pregnancy on the proliferative response of paternal derived splenocyte stimulated by pregnancy.Observe whether the pregnancy has a protective effect on the survival of grafted skin derived from paternal line by using the full thickness skin allograft model of allogeneic mice.Methods The possible mechanism of this effect was that the proportion of Tregs in CD4 + T cells during pregnancy increased gradually from 4.2% before pregnancy to about 6.8% after 8 days of pregnancy Decreased and gradually returned to baseline levels.MLR results showed that the stimulation of spleen cells from paternal origin, pregnancy group showed a significant low response to the control group, the average stimulation index was 7.8 and 13.6 (P <0.0 Quantitative PCR analysis showed that heme oxygenase-1 and indoleamine 2,3-dioxygenase mRNA were highly expressed in the placenta and spleen (P <0.05), and the mean Survival time in pregnancy and non-pregnancy group were 7.67 and 7.08 days, respectively, with no significant difference (P> 0.05) .Therefore, although the proportion of Treg with immunosuppressive function increased in pregnancy, Despite the lower MLR reactivity for paternal lineage-stimulated cells, the single pregnancy did not show statistically significant protective effects on the survival of grafts from paternal lines under the conditions of this study.