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本文研究了来自赣南7个县的柑橘绿霉病菌(Penicillium digitatum)种群对该地区常用杀菌剂抑霉唑、咪鲜胺、甲基硫菌灵和百可得的抗性频率、抗性水平和对抑霉唑的抗性分子机制。结果表明:病菌对抑霉唑和咪鲜胺存在基本一致的抗性;2011和2012年病菌种群对抑霉唑和咪鲜胺的抗性频率分别为82%和90%,平均抗性倍数为51.5倍,抗性分子机制均属于IMZ-R3,即CYP51B基因启动子区发生199 bp插入的突变;病菌种群对甲基硫菌灵的抗性频率分别为82%和91%;病菌种群对百可得均表现敏感。本研究为采后柑橘病害防治药剂选择提供了科学的依据。
In this paper, we studied the frequency and resistance level of Penicillium digitatum populations from 7 counties in southern Jiangxi province to the fungicides imazalil, prochloraz, thiophanate- And the molecular mechanism of resistance to imazalil. The results showed that there was basically consistent resistance to imazalil and prochloraz; the frequency of resistance to imazalil and prochloraz was 82% and 90% respectively in 2011 and 2012, and the average resistance multiple was 51.5-fold. The molecular mechanism of resistance was IMZ-R3, which was a 199-bp insertion mutation in the promoter region of CYP51B gene. The frequency of resistance to thiophanate-methyl was 82% and 91%, respectively. Available are sensitive performance. This study provides a scientific basis for the selection of pesticides for postharvest citrus disease prevention and control.