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本实验选择了３个ＤＮＡ探针，对胃癌病人最可能发生改变的３对染色体的基因位点进行了等位基因丢失研究。应用限制性片段长度多态性分析了３２例原发性胃癌及其癌周正常胃粘膜组织的等住基因变化。结果位于１７ｐ１３．３的探针ｐＹＮＺ２２在８例有信息意义的胃癌中检出３例杂合性丢失，位于７ｑ２１．１的探针ｐＧＹ３在２４例有信息意义的胃癌中检出２例，位于１２ｑ上的探针ｐＹＮＨ１５在１０例有信息意义的胃癌中检出１例。丢失频率分别为ｐＹＮＺ２２：３７．５％；ｐＧＹ３：８．３％；ｐＹＮＨ１５：１０％。结果提示，原发性胃癌的分子遗传学变化是复杂的，涉及到多个基因变化。
In this experiment, three DNA probes were selected to study allele loss in three pairs of chromosome loci that were most likely to change in patients with gastric cancer. Restriction fragment length polymorphism analysis was used to analyze the change of the isogenic gene in 32 cases of primary gastric cancer and its normal gastric mucosa. Results The pYNZ22 probe located at 17p13.3 detected loss of heterozygosity in 8 cases of gastric cancer with information significance. The probe pGY3 located at 7q21.1 was detected in 2 cases of 24 cases of gastric cancer with information significance. The probe pYNH15 on 12q detected 1 case in 10 cases of information-related gastric cancer. Loss frequency was pYNZ22:37.5%; pGY3:8.3%; pYNH15:10%. The results suggest that the molecular genetic changes in primary gastric cancer are complex and involve multiple genetic changes.