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目的:探讨G蛋白偶联受体30(GPR30)和磷酸化AKT(P-AKT)在子宫内膜腺癌发生发展中的作用及相互关系。方法:用免疫组化SP法检测10例增生期子宫内膜,49例子宫内膜增殖症,55例子宫内膜腺癌组织中GPR30和P-AKT的表达。结果:GPR30蛋白在子宫内膜腺癌、子宫内膜增殖症的阳性表达率(81.8%、67.3%)均明显高于增生期子宫内膜中的阳性表达率(20.0%);子宫内膜增殖症中GPR30蛋白在单纯型增生子宫内膜(SH)、复杂型增生子宫内膜(CH)和不典型增生子宫内膜(AH)的阳性表达率分别为30.0%(3/10),70.0%(14/20)和84.2%(16/19),AH和SH的差异有统计学意义(P=0.012)。P-AKT在子宫内膜腺癌、子宫内膜增殖症的阳性表达率(78.2%、71.4%)均显著高于增生期子宫内膜中的阳性表达率(20.0%);子宫内膜增殖症中P-AKT蛋白在SH、CH、AH的阳性表达率分别为40.0%(4/10),65.0%(13/20)和94.7%(18/19),AH与SH的差异有统计学意义(P=0.005)。子宫内膜腺癌GPR30、P-AKT蛋白阳性表达率与组织分化程度、FIGO分期及患者是否绝经有关,在中、低分化组(P=0.023;P=0.009)、FIGOⅡ～Ⅲ期(P=0.039;P=0.017)及绝经后(P=0.046;P=0.031)较高。肌层浸润较深的子宫内膜腺癌P-AKT蛋白表达水平高于肌层浸润较浅者(P=0.042)。子宫内膜腺癌组织中GPR30与P-AKT蛋白表达呈正相关(P<0.001)。结论:GPR30和P-AKT活化与子宫内膜癌的发生发展有关。
Objective: To investigate the role and relationship of G-protein coupled receptor 30 (GPR30) and phosphorylated AKT (P-AKT) in the development of endometrial adenocarcinoma. Methods: The expressions of GPR30 and P-AKT in 10 cases of proliferative endometrium, 49 cases of endometrial hyperplasia and 55 cases of endometrial adenocarcinoma were detected by immunohistochemical SP method. Results: The positive expression rates of GPR30 protein in endometrial adenocarcinoma and endometrial hyperplasia (81.8%, 67.3%) were significantly higher than those in proliferative endometrium (20.0%). The proliferation of endometrium The positive rates of GPR30 protein in patients with simple hyperplasia of endometrium (SH), complex hyperplasia of endometrium (CH) and atypical hyperplasia of endometrium (AH) were 30.0% (3/10) and 70.0% (14/20) and 84.2% (16/19) respectively. The difference between AH and SH was statistically significant (P = 0.012). P-AKT in endometrial adenocarcinoma, endometrial hyperplasia positive expression rate (78.2%, 71.4%) were significantly higher than that in proliferative endometrium (20.0%); endometrial hyperplasia The positive rates of P-AKT protein in SH, CH and AH were 40.0% (4/10), 65.0% (13/20) and 94.7% (18/19), respectively. The difference between AH and SH was statistically significant (P = 0.005). The positive expression rates of GPR30 and P-AKT protein in endometrial adenocarcinoma were correlated with histological grade, FIGO stage and whether the patients were menopausal. There was no significant difference between the two groups (P = 0.023; P = 0.009) 0.039; P = 0.017) and postmenopausal (P = 0.046; P = 0.031). The expression of P-AKT protein in deeper endometrial adenocarcinoma was higher than that in muscle myometrium (P = 0.042). The expression of GPR30 and P-AKT in endometrial adenocarcinoma was positively correlated (P <0.001). Conclusion: The activation of GPR30 and P-AKT is related to the occurrence and development of endometrial carcinoma.