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目的观察颈复康颗粒对颈椎动静力失衡大鼠颈椎间盘形态学改变的影响。方法将SD雄性大鼠随机分成对照组、模型组、颈复康颗粒组、莫比可组各12只,建立动静力失衡性大鼠颈椎间盘退变模型,给药4周后每组取4个标本2张连续切片,观察组织病理学改变,测量软骨终板非钙化层与钙化层厚度比值、软骨终板内血管芽数量,检测各组退变椎间盘血管内皮细胞生长因子(VEGF)、胰岛素样生长因子1(IGF-1)、转化生长因子β(TGF-β)表达。结果与对照组比较,模型组大鼠颈椎间盘出现退行性改变,软骨与椎体交界面血管芽数量减少,软骨终板非钙化层与钙化层比值下降,大鼠颈椎间盘中VEGF表达增加、IGF-1和TGF-β表达下降(P<0.01);与模型组比较,颈复康颗粒组血管芽数量增加(P<0.01),颈复康颗粒组及莫比可组椎间盘组织形态学改善(P<0.01或P<0.05),软骨终板非钙化与钙化层厚度比值增加(P<0.01),大鼠颈椎间盘中VEGF表达下降、IGF-1和TGF-β表达增加(P<0.01)。结论颈复康颗粒通过调节细胞因子VEGF、IGF-1、TGF-β的表达,改善大鼠颈椎间盘退变模型形态学的表现,从而减少椎间盘细胞外基质降解,延缓椎间盘退变。
Objective To observe the effect of Jingfukang Granules on the morphological changes of cervical intervertebral discs in rats with imbalanced dynamic and static cervical spine. Methods Male Sprague-Dawley rats were randomly divided into control group, model group, NJF group, and mobicon group, and 12 rats were used to establish a dynamic and static imbalance rat cervical disc degeneration model. After 4 weeks of dosing, each group took 4 rats. Two specimens were serially sectioned to observe histopathological changes. The ratio of the thickness of non-calcified and calcified layers in cartilage endplates and the number of vascular buds in cartilage endplates were measured. Each group of degenerated intervertebral discs was examined for vascular endothelial growth factor (VEGF) and insulin. Growth factor-1 (IGF-1), transforming growth factor-β (TGF-β) expression. Results Compared with the control group, the rat model showed degenerative changes in the cervical intervertebral disc, the number of vascular buds at the interface between the cartilage and the vertebral body decreased, the ratio of the calcified layer to the calcified layer of the cartilage endplate decreased, and the expression of VEGF in the rat cervical intervertebral disc increased. The expression of -1 and TGF-β decreased (P<0.01). Compared with the model group, the number of vascular buds increased in the Niaofukang Granule group (P<0.01). The morphological changes of the cervical intervertebral disc in the NJF group and Mobicor group were improved (P<0.01). P<0.01 or P<0.05), non-calcified and calcified layer thickness ratio of cartilage endplates increased (P<0.01), VEGF expression in cervical discs of rats decreased, and IGF-1 and TGF-β expression increased (P<0.01). Conclusion Jingfukang Granule can improve the morphology of rat model of cervical intervertebral disc degeneration by regulating the expression of cytokines VEGF, IGF-1 and TGF-β, thus reducing the degradation of extracellular matrix of intervertebral disc and delaying disc degeneration.