我们试验了妊娠早期绒毛贴片(壁)原位培养法制备染色体,并初步获得成功。取材:在无菌操作下,用注射器接上内径2～3mm的塑料管,经宫颁吸取妊娠9～10周的胚胎绒毛组织少许,注入无菌的采集小瓶内,尽快送细胞培养室。接种:方法1(贴片法):在无菌室或接种罩内,用含有抗菌素的Hank’s液清洗绒毛标本2～3次以除去血污,再以营养液(含25%小牛血清的RPMI1640)漂洗1次后,用无菌镊 We tested the chromosomal in situ preparation of villus patch (wall) in the first trimester of pregnancy and achieved initial success. Drawn: Aseptically connected to a plastic tube with an inner diameter of 2 ~ 3mm with a syringe. The villi of the embryo of the fetus grafted at 9 to 10 weeks of gestation was injected into a sterile collection vial for delivery to the cell culture chamber as soon as possible. Inoculation: Method 1 (patch method): Hank’s solution containing antibiotics is used to wash the villus 2 to 3 times in a sterile room or inoculation hood to remove bloodstains, and then a nutrient solution (RPMI1640 containing 25% fetal bovine serum) After rinsing 1 time with sterile tweezers