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目的:探讨提高单精子显微注射技术(intracytoplasmicsperminjection,ICSI)妊娠率的方法。方法:对2000年6月至2001年11月在我院生殖中心就诊的男性严重少精患者,对其妻采用常规超排卵方案,经阴道B超介导取卵,共获卵594个,采用改进的ICSI技术受精。结果:共有464个成熟卵,338个受精卵,受精率72.8%,优质胚胎率为80.8%,临床妊娠17例,妊娠率42.5%。结论:采用胚胎操作箱进行卵的操作,应用人工血清代用品代替自体血清,多精子制动分次注射,是否可以提高受精率、妊娠率,值得探讨。
Objective: To explore the method to improve the pregnancy rate of intracytoplasm microperitoneal injection (ICSI). Methods: From June 2000 to November 2001 in our hospital reproductive center for treatment of severe male patients with less serious, their wives using conventional superovulation program, vaginal B-mediated ovulation, a total of 594 eggs were obtained using Improved ICSI technology fertilization. RESULTS: A total of 464 mature eggs and 338 fertilized eggs were fertilized at a rate of 72.8%. The rate of high-quality embryos was 80.8%. Clinical pregnancy was performed in 17 cases and the pregnancy rate was 42.5%. Conclusion: The embryo operation box for egg manipulation, the use of artificial serum substitutes instead of autologous serum, multi-sperm brake injection, whether it can improve fertilization rate, pregnancy rate, worth exploring.