论文部分内容阅读
目的 观察地塞米松 (DM)对嗜酸细胞 (EOS)上白细胞介素 5受体α(IL 5Rα)、白细胞介素 3受体α(IL 3Rα)、粒 巨噬细胞集落刺激因子受体α(GM CSFRα)及共同β链 (βcR)mRNA表达的影响 ,探讨糖皮质激素促进哮喘EOS凋亡的机制。方法 18只健康豚鼠随机分为正常组、哮喘组、地塞米松组 ,每组 6只 ,以卵蛋白致敏激发制作哮喘豚鼠模型 ,密度梯度分离法分离支气管肺泡灌洗液(BALF)中的低密度EOS (HEOS)及正常密度EOS (NEOS) ,以末端脱氧核糖核苷酸转移酶介导的dUTP缺口末端标记法 (TUNEL)法检测细胞凋亡 ,原位杂交检测各受体mRNA表达 ,逆转录 多聚酶链式反应 (RT PCR)法检测EOSIL 5Rα、IL 3Rα相对含量。结果 HEOS及NEOS凋亡 ,哮喘组 (4 0± 2 0、3 0± 2 0 )与正常组 (8 0± 2 0、7 0± 2 0 )比较差异有显著性 (P <0 0 1) ,而细胞数哮喘组 (75 2± 12 6、5 0 7± 11 2 )与正常组 (4 8± 1 5、9 5± 2 6 )比较差异也有显著性 (P <0 0 1)。用DM 2 4h后不同密度EOS数量 (14 8± 8 3、2 0 0± 7 0 )与哮喘组比较差异有显著性 (P <0 0 1) ,DM组以HEOS下降为明显(P <0 0 5 ) ,BALF中EOS以NE0S为主 ,EOS凋亡DM组 (2 4 0± 5 0、2 2 0± 4 0 )与哮喘组比较差异有显著性 (P <0 0 1) ;EOS表达I
Objective To observe the effects of dexamethasone (DM) on eosinophils (EOS), interleukin 5 receptor α (IL 5Rα), interleukin 3 receptor α (IL 3Rα), granulocyte macrophage colony stimulating factor receptor α (GM CSFRα) and β-catenin (βcR) mRNA expression in asthmatic rats and explore the mechanism of glucocorticoid-induced apoptosis of EOS in asthmatic mice. Methods Eighteen healthy guinea pigs were randomly divided into normal group, asthma group and dexamethasone group, with 6 mice in each group. Asthmatic guinea pig models were induced by egg-protein sensitization. The density gradient was used to separate bronchoalveolar lavage fluid (BALF) Low density EOS (HEOS) and normal density EOS (NEOS) were detected by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) method. The mRNA expression of each receptor was detected by in situ hybridization. Reverse transcriptase polymerase chain reaction (RT PCR) method was used to detect the relative content of EOSIL 5Rα and IL 3Rα. Results The apoptosis of HEOS and NEOS in asthma group was significantly lower than that in normal group (40 ± 20,3 ± 20) and normal group (0 ± 20,7 ± 20) (P <0.01) (75 2 ± 12 6, 57 0 ± 11 2) in the asthma group (4 8 ± 1 5, 95 5 ± 2 6) compared with the normal group (P 0 01). The number of EOS at different density (14 8 ± 8 3, 200 ± 7 0) with DM 2 4h was significantly lower than that of the asthma group (P <0.01) (P <0.01). EOS expression in BALF was predominant in NEOS. EOS apoptosis in DM group was significantly higher than that in asthma group (P <0.001) I