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目的:设计一种载荷定量、精确、可控的应力性骨折动物模型构建新装置。方法:采用轴向周期性压缩加载的方法设计,整个装置由应力加载装置、线性促动器控制模块和数据实时采集与处理模块组成,其中应力加载装置主要由线性促动器、应力传感器、线性导轨、组织固定模块(包括动夹头和定夹头)和位移传感器组成。采用峰值压力为50 N的周期性压缩加载实验来检验该装置工作的可靠性,并使用该装置和Electro Force 3220万能材料试验机测量超高分子量聚乙烯(ultra-high molecular weight polyethylene,UHMWPE)小圆柱体标准材料的杨氏模量,通过对比来进行标定。结果:该装置可施加强度可控、时间可控、模式一致的周期性载荷,能够建立大鼠尺骨和胫骨2种应力性骨折动物模型,且工作准确性高。结论:该装置具有精确、科学和可靠的特点,所构建模型接近真实应力性骨折发生的情况,可为研究应力性骨折的发病机制、探索有价值的预防和治疗方法提供重要的动物模型平台支持。
Objective: To design a new device for animal model of stress fracture with quantitative, accurate and controllable load. Methods: The axial compression method was adopted. The whole device consisted of stress loading device, linear actuator control module and real-time data acquisition and processing module. The stress loading device consisted of a linear actuator, a stress sensor, a linear Rail, tissue fixed module (including moving chuck and fixed chuck) and the displacement sensor. Periodic compression loading experiments with a peak pressure of 50 N were used to verify the reliability of the operation of the device and the device was used with the Electro Force 3220 universal material testing machine to measure the ultra-high molecular weight polyethylene (UHMWPE) The Young’s modulus of the cylinder’s standard material is calibrated by comparison. Results: The apparatus can be applied with periodic load with controllable intensity, time control and consistent mode, and can establish animal models of two kinds of stress fractures of ulna and tibia in rats with high working accuracy. CONCLUSIONS: The device is accurate, scientific and reliable. The model is close to the real stress fractures and provides an important platform for animal models to study the pathogenesis of stress fractures and explore valuable preventive and therapeutic approaches .