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建立了反相离子对色谱法测定兔血清中二氯乙酸钠含量的方法。ＤＣＡ－Ｎａ在碱性条件下加热与水合肼定量生成稳定且有较强吸收系数的腙，以离子对色谱试剂ＩＰＲ－Ｂ_８为对离子，在ＺｏｒｂａｘＯＤＳ（４．６ｍｍ×２５０ｍｍ）柱上，以水－甲醇－ＩＰＲ－Ｂ_８（６４：３４：２，Ｖ／Ｖ）为流动相得到较好的分离，灵敏度０．０４ＡＵＦＳ，在２６５ｎｍ处测定，衍生物线性范围在０．６～５０．０μｇ／ｍｌ，ｒ＝０．９９９４，平均回收率为９８．０±３．６％，日内及日间精密度ＲＳＤ分别为２．６％和３．３％。以本法测定了ＤＣＡ－Ｎａ在兔体内的血药浓度及药代动力学参数。
A method for determination of sodium dichloroacetate in rabbit serum by reversed-phase ion-pair chromatography was established. DCA-Na was heated to hydrazine hydrate quantitatively under alkaline conditions to generate a stable and strong hydrazone with strong absorption coefficient. The ion pair chromatographic reagent IPR-B_8 was used as a counter ion on a Zorbax ODS (4.6 mm × 250 mm) column with water - methanol-IPR-B_8 (64:34: 2, V / V) as the mobile phase, the sensitivity was 0.04AUFS and the peak was at 265nm. The linear range of the derivative was 0.6 ~ 50.0μg / ml , R = 0.9994. The average recovery was 98.0 ± 3.6%. The intra-day and inter-day RSD were 2.6% and 3.3%, respectively. The DCA-Na concentration in plasma and pharmacokinetic parameters were determined by this method.