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Mung bean (Vigna radiata L.) is rich in D-chiro-inositol (DCI),vitexin,and isovitexin,which has beneficial effects on antidiabetic and inhibits the formation of advanced glycation end-products.In this study,near-infrared reflectance spectroscopy (NIRS) was used to predict the contents of DCI,vitexin,and isovitexin in mung bean.The spectra data were linearized with those determined by high-performance liquid chromatography (HPLC).The models for predicting the DCI,vitexin,and isovitexin contents in mung bean were developed using partial least-squares (PLS) algorithm.Cross-validation procedures indicated good correlations between HPLC data and NIRS predictions (R2=0.90 for DCI,R2=0.81 for vitexin,and R2=0.90 for isovitexin).The predictive contents of DCI,vitexin,and isovitexin ranged from 2.082 to 3.084％,1.277 to 1.307％,and 0.5998 to 0.6286％,respectively.The results showed that NIRS,a well-established and widely applied technique,could be applied to rapid detection of DCI,vitexin,and isovitexin contents in mung bean.