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目的研究乙型肝炎病毒(HBV)基因分型与前C基因及基本核心启动子(BCP)突变,为临床优化乙型肝炎治疗方案提供决策依据。方法征集98例未接受过抗病毒治疗的慢性乙型肝炎(CHB)患者为研究对象。通过乙肝表面抗原(HBsAg)基因测序进行基因分型。同时对前C基因及BCP进行测序分析。结果研究对象中HBV基因分型为:A:11例(11.2%);B:20例(20.4%);C:56例(57.1%);D:8例(8.2%);B/C混合型:3例(3.1%)。与其他基因型相比较,基因B和C型的患病率较高。基因型C的前C基因突变率为46.9%(46/98),较其他基因型的高,差异具有统计学意义(χ2=47.85,P<0.01);基因型C的BCP突变率为41.8%(41/98),较其他基因型的高,差异具有统计学意义(χ2=67.14,P<0.01)。结论 HBV基因分型以B、C基因为主。基因型C与BCP和前C基因突变密切相关。
Objective To study the genotypes of hepatitis B virus (HBV) and the mutations of pre-C gene and basic core promoter (BCP) in order to provide a basis for clinical treatment of hepatitis B patients. Methods A total of 98 patients with chronic hepatitis B (CHB) who did not receive antiviral therapy were enrolled in this study. Genotyping was performed by hepatitis B surface antigen (HBsAg) gene sequencing. At the same time, the pre-C gene and BCP were sequenced and analyzed. Results HBV genotypes in the study subjects were: A: 11 cases (11.2%); B: 20 cases (20.4%); C: 56 cases (57.1%); D: 8 cases Type: 3 cases (3.1%). Compared with other genotypes, genotypes B and C have a higher prevalence. The mutation rate of C gene in genotype C was 46.9% (46/98), which was significantly higher than other genotypes (χ2 = 47.85, P <0.01). The genotype C mutation rate was 41.8% (41/98), higher than other genotypes, the difference was statistically significant (χ2 = 67.14, P <0.01). Conclusion HBV genotypes mainly include B and C genes. Genotype C is closely related to BCP and pre-C gene mutations.