喉粘膜上皮是由复层鳞状上皮与假复层纤毛上皮组成，复层鳞状上皮是上皮增生、发育异常及癌的好发部位。体外喉粘膜重建可更好地了解喉细胞生物学特性，并对喉病的研究提供模式。传统的单层培养系统对研究不能提供一个满意的环境，因为体内上皮是复层的，与细胞外基质包括间质 Laryngeal mucosal epithelium is composed of stratified squamous epithelium and pseudostratified ciliated epithelium, stratified squamous epithelium is epithelial hyperplasia, dysplasia and cancer of the site of a good hair. In vitro laryngeal mucosal reconstruction provides a better understanding of laryngeal cell biology and provides a model for the study of laryngology. Traditional monolayer culture systems do not provide a satisfactory environment for the study because the epithelial cells in the body are stratified and interact with the extracellular matrix including the stroma