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利用新一代高通量测序技术平台Illumina Solexa GAⅡx对大岩桐花萼和幼叶进行转录组测序和数据从头组装及生物信息学分析,获得了长度大于100 bp的转录物22 821条;转录本总长度28.63Mb,N50长度1 548 bp,序列平均长度953 bp。转录物注释结果显示,15 053条有同源比对信息;7 768条无匹配序列信息,可能为大岩桐特有的基因序列。通过对花萼、幼叶中与激素代谢、信号转导相关的差异KEGG通路比较,推断在花萼中促进生长激素合成基因的表达比幼叶中强,激素信号转导组分基因的表达也比幼叶中强。
A total of 22 821 transcripts of more than 100 bp were obtained by sequencing and data analysis of the transcriptome and the bioinformatics analysis using the new generation high-throughput sequencing platform Illumina Solexa GAⅡx. The total transcript length 28.63Mb, N 545 bp in length and 953 bp in length. Transcript annotation results showed that there were 15 053 homologous alignment information; and 7 768 non-matching sequence information, which may be unique gene sequences of Euphorbiaceae. Compared with the differential KEGG pathway related to hormone metabolism and signal transduction in calyces and young leaves, it was inferred that the expression of growth hormone synthesis gene in calyx was stronger than that in young leaves, and the expression of hormone signal transduction component gene was stronger than that in young leaves.