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本研究旨在评价微剂量(37kBq)~(14)C-尿素呼气试验(~(14)C-UBT)对幽门螺杆菌(HP)感染的诊断效果。106例病人清晨空腹饮下37kBq~(14)C-尿素水溶液,海胺溶液吸收20分钟呼气CO_2mmol,液闪仪测定~(14)C放射性活性,结果以dpm/mmolCO_2表达。19例志愿者第二日重复试验。以Warthin-Starry组织染色和快速尿素酶试验作为参照标准,ROC分析法确定~(14)C-UBT最适判别值。结果得出~(14)C-UBT最适判别值为250dpm/mmol CO_2;实验对HP感染诊断的敏感性、特异性和准确性分别为98.3％(58/59)、98.7％(46/47)、和98.7％(104/106);实验重复性良好;体重校正对诊断效果无影响。研究结果提示微剂量~(14)C-UBT诊断HP感染具有高度的准确性,且简单和安全;试验无需试餐和加用非标记尿素,结果不需以体重校正。
The aim of this study was to evaluate the diagnostic value of C-UBT (~ (14) C-UBT) in Helicobacter pylori (HP) infection. One hundred and sixty patients underwent an early morning fasting drink of 37 kBq ~ (14) C-urea aqueous solution, and the ammonia solution was exhaled for 20 minutes and CO 2 mmol was exhaled. The radioactivity of ~ (14) C was measured by liquid scintillation counting and the result was expressed as dpm / mmolCO_2. Nineteen volunteers repeated the test on the second day. Warthin-Starry tissue staining and rapid urease test as a reference standard, ROC analysis to determine ~ (14) C-UBT optimal discriminant value. The results showed that the optimal discriminant value of ~ (14) C-UBT was 250 dpm / mmol CO 2. The sensitivity, specificity and accuracy of the test were 98.3% (58/59) and 98.7% (46/47) ), And 98.7% (104/106). The experiment was repeated with good reproducibility. Body weight correction had no effect on the diagnosis. The results suggest that the micro-dose 14 C-UBT diagnosis of HP infection with high accuracy, and simple and safe; test without test meal and add non-labeled urea, the results do not need to be corrected for body weight.